Efficient production of human α-amylase by a Bacillus brevis mutant (original) (raw)
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References
- Adachi T, Yamagata H, Tsukagoshi N, Udaka S (1989) Multiple and tandemly arranged promoters of the cell wall protein gene operon in Bacillus brevis 47. J Bacteriol 171:1010–1016
Google Scholar - Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
CAS PubMed Google Scholar - Brake AJ, Merryweather JP, Coit DG, Heberlein UA, Masiarz FR, Mullenbach GT, Urdea MS, Valenzuela P, Barr PJ (1984) α-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 81:4642–4646
Google Scholar - Gruber K, Tanahashi H, Tsuboi A, Tsukagoshi N, Udaka S (1988) Comparative study on the cell wall structure of protein-producing Bacillus brevis. FEMS Microbiol Lett 56:113–118
Google Scholar - Gryczan TJ, Contente S, Dubnau D (1978) Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J BBateriol 134:318–329
Google Scholar - Harris TJR (1983) Expression of eukaryotic genes in E. coli. In: Williamson GR (ed) Genetic engineering, vol 4. Academic Press, London, pp 127–185
Google Scholar - Hayakawa T, Toibana A, Marumoto R, Nakahama K, Kikuchi M, Fujimoto K, Ikehara M (1987) Expression of human lysosyme in an insoluble form in yeast. Gene 56:53–59
Article CAS PubMed Google Scholar - Himeno T, Imanaka T, Aiba S (1986) Protein secretion in Bacillus subtilis as influenced by the combination of signal sequence and the following mature portion. FEMS Microbiol Lett 35:17–21
Google Scholar - Horinouchi S, Weisblum B (1982) Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibiotics. J Bacteriol 150:804–814
Google Scholar - Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
PubMed Google Scholar - Miyashiro S, Enei H, Hirose Y, Udaka S (1980) Effects of glycine and l-isoleucine on protein production by Bacillus brevis no. 47. Agric Biol Chem 44:105–112
Google Scholar - Nakamura Y, Ogawa M, Nishide T, Emi M, Kasaki G, Himeno S, Matsubara K (1984) Sequences of cDNAs for human salivary and pancreatic α-amylases. Gene 28:263–270
Google Scholar - Nakamura Y, Sato T, Emi M, Miyanohara A, Nishide T, Matsubara K (1986) Expression of human salivary α-amylase gene in Saccharomyces cerevisiae and its secretion using the mammalian signal sequence. Gene 50:239–245
Google Scholar - Nishide T, Emi M, Nakamura Y, Matsubara K (1986) Corrected sequences of cDNAs for human salivary and pancreatic α-amylases. Gene 50:371–372
Google Scholar - Saito N (1973) A thermophilic extracellular α-amylase from Bacillus licheniformis. Arch Biochem Biophys 155:290–298
Google Scholar - Shiroza T, Nakagawa K, Tashiro N, Yamane K, Yanagi K, Yamasaki M, Tamura G, Saito H, Kawade Y, Taniguchi T (1985) Synthesis and secretion of biologically active mouse interferon-\ using a Bacillus subtilis α-amylase secretion vector. Gene 34:1–8
Google Scholar - Simons G, Remaut E, Allet B, Devos R, Fiers W (1984) High level expression of human interferon gamma in Escherichia coli under control of the P L promoter of bacteriophage lambda. Gene 28:55–64
Google Scholar - Smith RA, Funcan MJ, Moir DT (1985) Heterologous protein secretion from yeast. Science 229:1219–1224
Google Scholar - Takagi H, Kadowaki K, Udaka S (1989a) Screening and characterization of protein-hyperproducing bacteria without detectable exoprotease activity. Agric Biol Chem 53:691–699
Google Scholar - Takagi H, Miyauchi A, Kadowaki K, Udaka S (1989b) Potential use of Bacillus brevis HPD31 for the production of foreign proteins. Agric Biol Chem 53:2279–2280
Google Scholar - Takagi H, Kagiyama S, Kadowaki K, Tsukagoshi N, Udaka S (1989c) Genetic transformation of Bacillus brevis with plasmid DNA by electroporation. Agric Biol Chem 53:3099–3100
Google Scholar - Takahashi W, Yamagata H, Yamaguchi K, Tsukagoshi N, Udaka S (1983) Genetic transformation of Bacillus brevis 47, a protein-secreting bacterium. J Bacteriol 156:1130–1134
Google Scholar - Takao M, Morioka T, Yamagata H, Tsukagoshi N, Udaka S (1989) Production of swine pepsinogen by protein-producing Bacillus brevis carrying swine pepsinogen cDNA. Appl Microbiol Biotechnol 30:75–80
Google Scholar - Towbin H, Staechelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
CAS PubMed Google Scholar - Udaka S (1976) Screening for protein-producing bacteria. Agric Biol Chem 40:523–528
Google Scholar - Wang L-F, Wong S-L, Lee S-G, Kalyan NK, Hung PP, Hilliker S, Doi RH (1988) Expression and secretion of human atrial natriuretic α-factor in Bacillus subtilis using the subtilisin signal peptide. Gene 69:39–47
Google Scholar - Yamada H, Tsukagoshi N, Udaka S (1981) Morphological alterations of cell wall concomitant with protein release in a protein-producing bacterium, Bacillus brevis 47. J Bacteriol 148:322–332
Google Scholar - Yamagata H, Adachi T, Tsuboi A, Takao M, Sasaki T, Tsukagoshi N, Udaka S (1987) Cloning and characterization of the 5' region of the cell wall protein gene operon in Bacillus brevis 47. J Bacteriol 169:1239–1245
Google Scholar - Yamagata H, Nakahama K, Suzuki Y, Kakinuma A, Tsukagoshi N, Udaka S (1989) Use of Bacillus brevis for efficient synthesis and secretion of human epidermal growth factor. Proc Natl Acad Sci USA 86:3589–3593
Google Scholar