Characterization of a bacteriocin produced by Streptococcus thermophilus ST134 (original) (raw)
Abstract
A pH-dependent adsorption/desorption technique was used to screen Streptococcus thermophilus strains for the production of bacteriocins. Agar-diffusion tests with S. thermophilus strains as targets identified 13 out of 41 strains as producers of antibacterial activity. Thermophilin A, the bacteriocin-like substance present in the culture supernatant of S.thermophilus ST134 was purified to homogeneity by ammonium sulfate precipitation and ion-exchange chromatography, followed by ultrafiltration. Thermophilin A is a relatively heat-stable and apparently glycosylated bacteriocin with a bactericidal mode of action against sensitive cells.
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References
- Andersson RE, Daeschel MA, Hassan HM (1988) Antibacterial activity of plantarian SIK-83, a bacteriocin produced by Lactobacillus plantarum. Biochimie 70:381–390
Google Scholar - Cilano L, Bossi MG, Carini S (1990) Bacteriocin production by Streptococcus thermophilus. Microbiol Aliment Nutr 8:21–30
Google Scholar - Daba H, Pandian S, Gosselin JF, Simard RE, Huang J, Lacroix C(1991) Detection and activity of a bacteriocin produced by Leuconostoc mesenteroides. Appl Environ Microbiol 57:3450–3455
Google Scholar - Davey GP (1984) Plasmid associated with diplococcin production in Streptococcus cremoris 346. Appl Environ Microbiol 48:895–896
Google Scholar - Farkas-Himsley H (1980) Bacteriocins, are they broad spectrum antibiotics? J Antimicrob Chemother 6:224–227
Google Scholar - Heap HA, Lawrence RC (1976) The selection of starter strains for cheese making. J Dairy Sci Technol 11:16–20
Google Scholar - Jimenez-Diaz R, Rios-Sanchez RM, Desmazeaud M, Ruiz-Barbra JL, Piard JC (1993) Plantaricins S and T, two new bacteriocins produced by Lactobacillus plantarum LPC010 isolated from a green olive fermentation. Appl Environ Microbiol 59:1416–1424
Google Scholar - Joerger MC, Klaenhammer TR (1986) Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481. J Bacteriol 167:439–446
Google Scholar - Joerger MC, Klaenhammer TR (1990) Cloning, expression, and nucleotide sequence of the Lactobacillus helveticus 481 gene encoding the bacteriocin helveticin J. J Bacteriol 177:6339–6347
Google Scholar - Kozak W, Bardowski J, Dobrzanski WT (1978) Lactostrepcins, acid bacteriocins produced by lactic streptococci. J Dairy Res 45:247–257
Google Scholar - Laemmli UK (1970) Cleavage of structrual proteins during the assembly of the head bacteriophage T4. Nature 227:680–685
PubMed Google Scholar - Lewus CB, Sun S, Montville TJ (1992) Production of an amylasesensitive bacteriocin by an atypical Leuconostoc paramesenteroides strain. Appl Environ Microbiol 58:143–149
CAS Google Scholar - Mayr-Harting A, Hedges AJ, Berkeley RCW (1972) Methods for studying bacteriocins. Methods Microbiol 7:315–422
Google Scholar - Mortvedt CI, Nes IF (1990) Plasmid associated bacteriocin production by a Lactobacillus sake strain. J Gen Microbiol 136:1601–1607
Google Scholar - Nettles CE, Barefoot SF (1993) Biochemical and genetic characteristics of bacteriocins of food-associated lactic acid bacteria. J Food Prot 56:338–356
CAS Google Scholar - Pucci MJ, Vedamuthu ER, Kinka BS, Vanderbergh PA (1988) Inhibition of Listeria monocytogenes by using bacteriocin PA-1 produced by Pediococcus acidilactici. Appl Environ Microbiol 54:2349–2353
Google Scholar - Ray SS, Johnson MC, Ray B (1989) Bacteriocin plasmids of Pedicoccus acidilactici. J Ind Microbiol 4:163–171
Google Scholar - Scherwitz KM, Baldwin KA, McKay LL (1983) Plasmid linkage of a bacteriocin-like substance in Streptococcus lactis subsp. diacetylactis strain WM4: transferability to Streptococcus lactis. Appl Environ Microbiol 45:1506–1512
Google Scholar - Schillinger U, Lucke FK (1989) Antibacterial activity of Lactobacillus sake isolated from meat. Appl Environ Microbiol 55:1901–1906
Google Scholar - Schev F, Lalazar A, Henis Y, Juven BJ (1993) Purification, partial characterization and plasmid-linkage of pediocin SJ-1, a bacteriocin produced by Pediococcus acidilactici. J Appl Bacteriol 74:67–77
Google Scholar - Somkuti GA, Steinberg DH (1986) Distribution and analysis of plasmids in Streptococcus thermophilus. J Ind Microbiol 1:157–163
Google Scholar - Somkuti GA, Steinberg DH (1988) Genetic transformation of Streptococcus thermophilus by electroporation. Biochimie 70:579–585
Google Scholar - Tagg JR, McGiven A R (1971) Assay system for bacteriocins. Appl Microbiol 21:943
CAS PubMed Google Scholar - Tagg JR, Dajani AS, Wannamaker LW (1976) Bacteriocins of gram positive bacteria. Bacteriol Rev 40:722–756
CAS PubMed Google Scholar - West CA, Warner PJ (1988) Plantacin B, a bacteriocin produced by Lactobacillus plantarum NCDO 1193. FEMS Microbiol Lett 49:163–165
Google Scholar - Vaughan EE, Daly C, Fitzgerald GF (1992) Identification and characterization of helveticin V-1829, a bacteriocin produced by Lactobacillus helveticus 1829. J Appl Bacteriol 73:299–308
Google Scholar - Yang R, Johnson MC, Ray B (1992) Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl Environ Microbiol 58:3355–3359
CAS PubMed Google Scholar
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Authors and Affiliations
- U.S. Department of Agriculture, ARS, Eastern Regional Research Center, 600 E. Mermaid Lane, 19118, Philadelphia, PA, USA
D. J. Ward & G. A. Somkuti
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- D. J. Ward
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Ward, D.J., Somkuti, G.A. Characterization of a bacteriocin produced by Streptococcus thermophilus ST134.Appl Microbiol Biotechnol 43, 330–335 (1995). https://doi.org/10.1007/BF00172834
- Received: 10 June 1994
- Revised: 25 August 1994
- Accepted: 12 September 1994
- Issue Date: June 1995
- DOI: https://doi.org/10.1007/BF00172834