Enzyme stabilization be ectoine-type compatible solutes: protection against heating, freezing and drying (original) (raw)

Summary

The aim of this study was to elucidate the protective effect of the new compatible solutes, ectoine and hydroxyectoine, on two sensitive enzymes (lactic dehydrogenase, phosphofructokinase). The solutes tested also included (for reasons of comparison) other compatible solutes such as glycine betaine and a number of disaccharides (sucrose, trehalose, maltose). All compatible solutes under investigation displayed remarkable stabilizing capabilities. However, the degree of protection depended on both the type of solute chosen and the enzyme used as a test system. The most prominent protectants were trehalose, ectoine and hydroxyectoine, which are very often found in nature (singly or in combinationn) as part of the compatible solute “cocktail” of moderately halophilic eubacteria.

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Authors and Affiliations

1. Institut für Mikrobiologie and Biotechnologie, Rheinische Friedrich-Wilhelms-Universität, Meckenheimer Alle 168, W-5300, Bonn 1, Federal Republic of Germany
   Karin Lippert & Erwin A. Galinski

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1. Karin Lippert
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2. Erwin A. Galinski
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Offprint request to: E. A. Galinski

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Lippert, K., Galinski, E.A. Enzyme stabilization be ectoine-type compatible solutes: protection against heating, freezing and drying.Appl Microbiol Biotechnol 37, 61–65 (1992). https://doi.org/10.1007/BF00174204

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• Received: 09 August 1991
• Accepted: 19 December 1991
• Issue Date: April 1992
• DOI: https://doi.org/10.1007/BF00174204

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