Spermidine biosynthesis as affected by osmotic stress in oat leaves (original) (raw)
Abstract
A new assay for the evaluation of spermidine (Spd) synthase activity was developed. It involves a coupled reaction and avoids the use of decarboxylated S-adenosylmethionine, which is unstable and not easily available. This assay was applied to assess changes in enzyme activity in oat leaves subjected to osmotic stress in the dark. The results indicate that osmotically-induced putrescine (Put) accumulation in cereals results not only from the activation of the arginine decarboxylase pathway, but also from the inhibition of the activity of Spd synthase, the enzyme which catalyzes the transformation of Put to Spd. Other possibilities which could contribute to the decline of Spd and spermine levels under osmotic stress are also discussed.
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Abbreviations
ADC:
arginine decarboxylase
Dap:
diaminopropane
DFMA:
α-difluoromethylarginine
MGBG:
methylglyoxal-bis-guanylhydrazone
MTA:
5′-deoxy-5′-methylthioadenosine
ODC:
ornithine decarboxylase
PA:
polyamines
PAO:
polyamine oxidase
PCA:
perchloric acid
PLP:
pyridoxal phosphate
Put:
putrescine
SAM:
S-adenosylmethionine
dSAM:
decarboxylated S-adenosylmethionine
SAMDC:
S-adenosylmethionine decarboxylase
Spd:
spermidine
Spm:
spermine
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Authors and Affiliations
- Unitat de Fisiologia Vegetal, Facultat de Farmàcia, Universitat de Barcelona, Av. Diagonal 643, 08028, Barcelona, Spain
A. F. Tiburcio - Department of Biology, Yale University, P.O. Box 6666, 06511, New Haven, CT, USA
R. Kaur-Sawhneyz & A. W. Galston
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- A. F. Tiburcio
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Tiburcio, A.F., Kaur-Sawhneyz, R. & Galston, A.W. Spermidine biosynthesis as affected by osmotic stress in oat leaves.Plant Growth Regul 13, 103–109 (1993). https://doi.org/10.1007/BF00207599
- Received: 02 February 1992
- Accepted: 09 October 1992
- Issue Date: May 1993
- DOI: https://doi.org/10.1007/BF00207599