An RFLP linkage map for loblolly pine based on a three-generation outbred pedigree (original) (raw)

Abstract

A genetic linkage map for loblolly pine (Pinus taeda L.) was constructed using segregation data from a three-generation outbred pedigree consisting of four grandparents, two parents, and 95 F2 progeny. The map was based predominantly on restriction fragment length polymorphism (RFLP) loci detected by cDNA probes. Sixty-five cDNA and three genomic DNA probes revealed 90 RFLP loci. Six polymorphic isozyme loci were also scored. One-fourth (24%) of the cDNA probes detected more than 1 segregating locus, an indication that multigene families are common in pines. As many as six alleles were observed at a single segregating locus among grandparents and it was not unusual for the progeny to segregate for three or four alleles per locus. Multipoint linkage analysis placed 73 RFLP and 2 isozyme loci into 20 linkage groups; the remaining 17 RFLP and 4 isozyme loci were unlinked. The mapped RFLP probes provide a new set of codominant markers for genetic analyses in loblolly pine.

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Authors and Affiliations

1. Division of Forestry/CSIRO, Queen Victoria Terrace, P.O. Box 4008, 2600, Canberra, ACT, Australia
   M. E. Devey
2. School of Medicine, Oregon Health Sciences, 97201, Portland, OR, USA
   T. A. Fiddler
3. Department of Forestry, North Carolina State University, 27695, Raleigh, NC, USA
   B. H. Liu
4. Department of Crop and Soil Sciences, Oregon State University, 97331, Corvallis, OR, USA
   S. J. Knapp
5. Pacific Southwest Research Station, Institute of Forest Genetics, USDA/Forest Service, P.O. Box 245, 94701, Berkeley, CA, USA
   D. B. Neale

Authors

1. M. E. Devey
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2. T. A. Fiddler
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3. B. H. Liu
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4. S. J. Knapp
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5. D. B. Neale
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Communicated by P. M. A. Tigerstedt

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Devey, M.E., Fiddler, T.A., Liu, B.H. et al. An RFLP linkage map for loblolly pine based on a three-generation outbred pedigree.Theoret. Appl. Genetics 88, 273–278 (1994). https://doi.org/10.1007/BF00223631

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• Received: 01 August 1993
• Accepted: 09 September 1993
• Issue Date: June 1994
• DOI: https://doi.org/10.1007/BF00223631

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