Thy-1 is a neuronal and glial surface antigen which interacts with matrix proteins and plasminogen activator (original) (raw)

Summary

Thy-1 antigen is expressed at high levels in the thymus and in adult brain of rodents however its function remains undetermined. We report that immobilised Thy-1 binds laminin, fibronectin and the less active precursor form of the tissue type plasminogen activator (t-PA) yet it does not bind urokinase. The incorporation of serine protease inhibitors within the experimental procedures suggested that Thy-1 bound to the lysine-containing, protein-binding domain of t-PA thus leaving the active site available to interact with other proteins. By using an immunocytochemical approach designed to maximally preserve Thy-1 antigenicity, we were able to demonstrate that in the adult rat peripheral nervous system (PNS) Thy-1 was seen to co-localise with laminin on the Schwann cell membranes and accumulated at the nodes of Ranvier within sciatic nerve. The only neuronal structures to express Thy-1 within the PNS were the unmyelinated nerve fibres. In the adult rat central nervous system (CNS), the most distinct and novel association of Thy-1 was its presence along the myelin forming glial cells and their fibres.

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Authors and Affiliations

1. Recombinant DNA Laboratory, University of Helsinki, Valimotie 7, SF-00380, Helsinki, Finland
   P. Liësi
2. Department of Virology, University of Helsinki, Haartmaninkatu 3, SF-00290, Helsinki, Finland
   E. -M. Salonen & A. Vaheri
3. SCI Research Medical Centre, Veterans Administration, 1400 Veterans of Foreign Wars Parkway, 02132, West Roxbury, MA, USA
   D. Dahl
4. MRC Molecular Genetics Unit, Addenbrookes Hospital, Hills Road, CB2 2QH, Cambridge, UK
   S. -J. Richards

Authors

1. P. Liësi
2. E. -M. Salonen
3. D. Dahl
4. A. Vaheri
5. S. -J. Richards

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Liësi, P., Salonen, E.M., Dahl, D. et al. Thy-1 is a neuronal and glial surface antigen which interacts with matrix proteins and plasminogen activator.Exp Brain Res 79, 642–650 (1990). https://doi.org/10.1007/BF00229332

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• Received: 27 April 1989
• Accepted: 28 September 1989
• Issue date: March 1990
• DOI: https://doi.org/10.1007/BF00229332

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