Calcium binding proteins and neuropeptides as molecular markers of GABAergic interneurons in the cat visual cortex (original) (raw)

Summary

In the cat visual cortex, almost all parvalbumin-positive cells are GABAergic, and about 80% of the calbindin D-28K-positive neurons are also GABA-immunoreactive. About 37% of the GABAergic neurons contain parvalbumin and a smaller fraction (about 18%) contains calbindin. Furthermore, parvalbumin and calbindin are localized in two separate neuronal populations in the cat visual cortex, suggesting that two GABAergic populations can be distinguished, one containing parvalbumin and one containing calbindin. Double staining for parvalbumin and neuropeptides (CCK, SRIF and NPY), revealed no double-labeled cells, with the exception of a few SRIF- and parvalbumin-positive neurons. These results show that cortical GABAergic cells can be differentiated on basis of their calcium binding protein and neuropeptide immunoreactivity.

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Authors and Affiliations

1. Laboratory of Neuroendocrinology and Immunological Biotechnology, Zoological Institute, Catholic University Leuven, B-3000, Naamsestr., Leuven, Belgium
   H. Demeulemeester, L. Arckens & F. Vandesande
2. Laboratory of Neuro- and Psychophysiology, Catholic University Leuven Campus, B-3000, Gasthuisberg, Leuven, Belgium
   H. Demeulemeester, L. Arckens & G. A. Orban
3. Department of Pediatrics, Division of Clinical Chemistry, University of Zürich, CH-8032, Zürich, Switzerland
   C. W. Heizmann
4. Laboratory of Histology, Faculty of Medicine, University of Brussels, B-1000, Brussels, Belgium
   R. Pochet

Authors

1. H. Demeulemeester
2. L. Arckens
3. F. Vandesande
4. G. A. Orban
5. C. W. Heizmann
6. R. Pochet

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Demeulemeester, H., Arckens, L., Vandesande, F. et al. Calcium binding proteins and neuropeptides as molecular markers of GABAergic interneurons in the cat visual cortex.Exp Brain Res 84, 538–544 (1991). https://doi.org/10.1007/BF00230966

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• Received: 30 January 1990
• Accepted: 02 July 1990
• Issue date: April 1991
• DOI: https://doi.org/10.1007/BF00230966

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