Quantitation of Na+/K+-ATpase and glucose transporter isoforms in rat adipocyte plasma membrane by immunogold labeling (original) (raw)
References
Anderson, R.G.W., Kamen, B.A., Rothberg, K.G., Lacey, S.W. 1992. Potocytosis: sequestration and transport of small molecules by caveolae. Science255:410–413 Google Scholar
Birnbaum, M.J. 1989. Identification of a novel gene encoding an insulin-responsive glucose transporter protein. Cell57:305–315 Google Scholar
Brodsky, J.L. 1990_a_. Characterization of the (Na++K+)-ATPase from 3T3-F442A fibroblasts and adipocytes. J. Biol. Chem.265:10458–10465 Google Scholar
Brodsky, J.L. 1990_b_. Insulin activation of brain Na-K-ATPase is mediated by α2-form enzyme. Am. J. Physiol.258:C812-C817 Google Scholar
Buxbaum, E., Schoner, W. 1991. Phosphate binding and ATPbinding sites coexist in Na+/K+-transporting ATPase, as demonstrated by the inactivating MgPO4 complex analogue Co (NH3)4PO4. Eur. J. Biochem.195:407–419 Google Scholar
Clausen, T. 1986. Regulation of active Na+-K+ transport in skeletal muscle. Physiol. Rev.66:542–580 Google Scholar
Clausen, T., Hansen, O. 1974. Ouabain binding and Na+-K+ transport in rat muscle cells and adipocytes. Biochim. Biophys. Acta345:387–404 Google Scholar
Clausen, T., Hansen, O. 1977. Active Na/K transport and the rate of ouabain binding. The effect of insulin and other stimuli on skeletal muscle and adipocytes. J. Physiol.270:415–430 Google Scholar
Craig, W.S. 1988. Determination of quaternary structure of an active enzyme using chemical cross-linking with glutaraldehyde. In: Methods in Enzymology. B. Fleischer and S. Fleischer, editors Vol. 156, pp. 333–345. Academic, New York Google Scholar
Cushman, S.W., Wardzala, L.J. 1980. Potential mechanism of insulin action on glucose transport in the isolated rat adipose cell. J. Biol. Chem.255:4758–4762 Google Scholar
Emanuel, J.R., Garetz, S., Stone, L., Levenson, R. 1987. Differential expression of Na+,K+-ATPase α- and β-subunit mRNAs in rat tissues and cell lines. Proc. Natl. Acad. Sci. USA84:9030–9034 Google Scholar
Erlij, D., Grinstein, S. 1976. The number of sodium ion pumping sites in skeletal muscle and its modification by insulin. J. Physiol.259:13–31 Google Scholar
Felsenfeld, D.P., Sweadner, K.J. 1988. Fine specificity mapping and topography of an isozyme-specific epitope of the Na,KATPase catalytic subunit. J. Biol. Chem.263:10932–10942 Google Scholar
Handberg, A., Kayser, L., Høyer, P.E., Vinten, J. 1992. A substantial part of GLUT-1 in crude membranes from muscle originates from perineurial sheaths. Am. J. Physiol.262:E721-E727 Google Scholar
Haney, P.M., Slot, J.W., Piper, R.C. James, D.E., Mueckler, M. 1991. Intracellular targeting of the insulin-regulatable glucose transporter (GLUT4) is isoform specific and independent of cell type. J. Cell Biol.114:689–699 Google Scholar
Herrera, V.L.M., Emanuel, J.R., Ruiz-Opazo, N., Levenson, R., Nadal-Ginard, B. 1987. Three differentially expressed Na,KATPase alpha subunit isoforms: structural and functional implications. J. Cell Biol.105:1855–1865 Google Scholar
Hootman, S.R., Ernst, S.A. 1988. Estimation of Na/K-pump numbers and turnover in intact cells with 3H-ouabain. In: Methods in Enzymology. B. Fleischer and S. Fleischer, editors Vol. 156, pp. 213–229. Academic, New York Google Scholar
Horisberger, M. 1981. Colloidal gold: A cytochemical marker for light and fluorescent microscopy and for transmission and scanning electron microscpy. Scan. Electron Micros.2:9–31 Google Scholar
Hsu, Y.-M., Guidotti, G. 1991. Effects of hypokalemia on the properties and expression of the (Na+,K+)-ATPase of rat skeletal muscle [published erratum appears in J. Biol. Chem. 1991, 266:7962]. J. Biol. Chem.266:427–433 Google Scholar
Hundal, H.S., Marette, A., Mitsumoto, Y., Ramlal, T., Blostein, R., Klip, A. 1992. Insulin induces translocation of the alpha 2 and beta 1 subunits of the Na+/K+-ATPase from intracellular compartments to the plasma membrane in mammalian skeletal muscle. J. Biol. Chem.267:5040–5043 Google Scholar
Jacobson, B.S., Cronin, J., Branton, D. 1978. Coupling polylysine to glass beads for plasma membrane isolation. Biochim. Biophys. Acta506:81–96 Google Scholar
James, D.E., Brown, R., Navarro, J., Pilch, P.F. 1988. Insulin regulatable tissues express a unique insulin sensitive glucose transport protein. Nature333:183–185 Google Scholar
Jensen, J., Nørby, J.G. 1988. Membrane-bound Na,K-ATPase: target size and radiation inactivation size of some of its enzymatic reactions. J. Biol. Chem.263:18063–18070 Google Scholar
Kashgarian, M., Biemesderfer, D. 1988. Preparation and use of monoclonal antibodies to Na/K-ATPase. In: Methods in Enzymology. B. Fleischer and S. Fleischer, editor Vol. 156, pp. 392–417, Academic, New York Google Scholar
Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227:680–685 PubMed Google Scholar
Liang, S.-M., Winter, C.G. 1977. Digitonin-induced changes in subunit arrangement in relation to some in vitro activities of the (Na+,K+)-ATPase. J. Biol. Chem.252:8278–8284 Google Scholar
Lowry, O.K., Rosebrough, N.J., Farr, A.L., Randall, R.J. 1951. Protein measurement with the folin reagent. J. Biol. Chem.193:265–275 CASPubMed Google Scholar
Lytton, J. 1985. Insulin affects the sodium affinity of the rat adipocyte (Na+,K+)-ATPase. J. Biol. Chem.260:10075–10080 Google Scholar
Lytton, J., Lin, J.C., Guidotti, G. 1985. Identification of two molecular forms of (Na+,K+)-ATPase in rat adipocytes. Relation to insulin stimulation of the enzyme. J. Biol. Chem.260:1177–1184 Google Scholar
Mayahara, H., Ogawa, O. 1988. Histochemical localization of Na+,K+-ATPase. In: Methods in Enzymology. B. Fleischer and S. Fleischer, editors. Vol. 156, pp. 417–430, Academic, New York Google Scholar
McGill, D.L. 1991. Characterization of the adipocyte ghost (Na+,K+) pump. Insights into the insulin regulation of the adipocyte (Na+,K+) pump. J. Biol. Chem.266:15817–15823 Google Scholar
McGill, D.L., Guidotti, G. 1991. Insulin stimulates both the α1 and the α2 isoforms of the rat adipocyte (Na+,K+) ATPase. Two mechanisms of stimulation. J. Biol. Chem.266:15824–15831 Google Scholar
McKeel, D.W., Jarett, L. 1970. Preparation and characterization of a plasma membrane fraction from isolated fat cells. J. Cell Biol.44:417–432 Google Scholar
Nørby, J.G., Jensen, J. 1991. Functional significance of the oligomeric structure of the Na/K-pump from radiation inactivation and ligand binding. In: The Sodium Pump: Structure, Mechanism, and Regulation, pp. 174–188. The Rockefeller University, New York Google Scholar
O'Connor, C.G., Ashman, L.K. 1982. Application of the nitrocellulose transfer technique and alkaline phosphatase conjugated anti-immunoglobulin for determination of the specificity of monoclonal antibodies to protein mixtures. J. Immunol. Methods54:267–273 Google Scholar
Ogawa, K.S., Fujimoto, K., Ogawa, K. 1986. Ultracytochemical studies of adenosine nucleotidases in rat aortic endothelial and smooth mucsle cells. Acta. Histochem. Cytochem.19:601–620 Google Scholar
Omatsu-Kanbe, M., Kitasato, H. 1990. Insulin stimulates the translocation of Na+/K+-dependent ATPase molecules from intracellular stores to the plasma membrane in frog skeletal muscle. Biochem. J.272:727–733 Google Scholar
Peterson, G.L. 1977. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem.83:346–356 Google Scholar
Resh, M.D. 1982_a_. Insulin action on the (Na+,K+)ATPase. Ph.D. Thesis, pp. 451–464. Harvard University, Cambridge, MA Google Scholar
Resh, M.D. 1982_b_. Quantitation and characterization of the (Na+,K+)-adenosine triphosphatase in the rat adipocyte plasma membrane. J. Biol. Chem.257:11946–11952 Google Scholar
Resh, M.D. 1982_c_. Development of insulin responsiveness of the glucose transporter and the (Na+,K+)-adenosine triphosphatase during in vitro adipocyte differentiation. J. Biol. Chem.257:6978–6986 Google Scholar
Resh, M.D. 1983. Insulin activation of (Na+,K+)-adenosinetriphosphatase exhibits a temperature-dependent lag time. Comparison to activation of the glucose transporter. Biochemistry22:2781–2784 Google Scholar
Resh, M.D. 1988. Identification and quantitation of Na,K-ATPase by back-door phosphorylation. In: Methods in Enzymology. B. Fleischer and S. Fleischer, editors. Vol. 156, pp. 119–126. Academic, New York Google Scholar
Resh, M.D., Nemenoff, R.A., Guidotti, G. 1980. Insulin stimulation of (Na+,K+)-adenosine triphosphatase-dependent 86Rb+ uptake in rat adipocytes. J. Biol. Chem.255:10938–10945 Google Scholar
Robinson, L.J., Pang, S., Harris, D.S., Heuser, J., James, D.E. 1992. Translocation of the glucose transporter (GLUT4) to the cell surface in permeabilized 3T3-L1 adipocytes: Effects of ATP, insulin, and GTPγS, and localization of the GLUT4 to clathrin lattices. J. Cell. Biol.117:1181–1196 Google Scholar
Russo, J.J., Manuli, M.A., Ismail-Beigi, F., Sweadner, K.J., Edelman, I.S. 1990. Na+,K+-ATPase in adipocyte differentiation in culture. Am. J. Physiol.259:C968-C977 Google Scholar
Schmitt, C.A., McDonough, A.A. 1986. Developmental and thyroid hormone regulation of two molecular forms of Na/K, ATPase in brain. J. Biol. Chem.261:10439–10444 Google Scholar
Skou, J.C. 1988. Overview: The Na/K-pump. In: Methods in Enzymology. B. Fleischer and S. Fleischer, editors. Vol. 156, pp. 1–29. Academic, New York Google Scholar
Slot, J.W., Posthuma, G., Chang, L.Y., Crapo, J.D., Geuze, H.J. 1989. Quantitative aspects of immunogold labeling in embedded and in nonembedded sections. Am. J. Anat.185:271–281 Google Scholar
Slot, J.W., Geuze, H.J. 1985. A new method of preparing gold probes for multiple-labeling cytochemistry. Eur. J. Cell Biol.38:87–93 Google Scholar
Smith, R.M., Charron, M.J., Shah, N., Lodish, H.F., Jarett, L. 1991. Immunoelectron microscopic demonstration of insulinstimulated translocation of glucose transporters to the plasma membrane of isolated rat adipocytes and masking of the carboxyl-terminal epitope of intracellular GLUT4. Proc. Natl. Acad. Sci. USA88:6893–6897 Google Scholar
Suzuki, K., Kono, T. 1980. Evidence that insulin causes translocation of glucose transport activity to the plasma membrane from an intracellular storage site. Proc. Natl. Acad. Sci. USA77:2542–2545 Google Scholar
Sweadner, K.J. 1989. Isozymes of the Na+/K+-ATPase. Biochim. Biophys. Acta.988:185–220 Google Scholar
Tranum-Jensen, J. 1988. Electron microscopy: Assays involving negative staining. In: Methods in Enzymology. S. Harshman, editor. Vol. 165, pp. 357–374. Academic, New York Google Scholar
Tranum-Jensen, J., Vinten, J., Ploug, T. 1988. The anatomy of the cytoplasmic rim and the plasma membrane of rat epididymal fat cells. J. Ultrastructure Mol. Structure Res.100:293 Google Scholar
Urayama, O., Shutt, H., Sweadner, K.J. 1989. Identification of three isozyme proteins of the catalytic subunit of the Na,KATPase in rat brain. J. Biol. Chem.264:8271–8280 Google Scholar
Urayama, O., Sweadner, K.J. 1988. Ouabain sensitivity of the α3 isozyme of rat Na,K-ATPase. Biochem. Biophys. Res. Commun.156:796–800 Google Scholar
Vinten, J., Gliemann, J., Østerlind, J. 1976. Exchange of 3-Omethylglucose in isolated fat cells. Concentration dependence and effect of insulin. J. Biol. Chem.251:794–800 Google Scholar
Vinten, J., Tranum-Jensen, J. 1991. Immunogold labeling of glucose transporters in adipocyte plasma membranes. Diabetologia34:A12 (Abstr.) Google Scholar
Yang, J., Clark, A.E., Kozka, I.J., Cushman, S.W., Holman, G.D. 1992. Development of an intracellular pool of glucose transporters in 3T3-L1 cells. J. Biol. Chem.267:10393–10399. Google Scholar