Classification of virulent bacteriophages of Streptococcus salivarius subsp. thermophilus isolated from yoghurt and Swiss-type cheese (original) (raw)

Summary

Twelve isometric-headed bacteriophages virulent against Streptococcus salivarius subsp. thermophilus were differentiated into three subgroups by analysis of the phage genomes and the structural proteins. Subgroup I is composed of two phages (P6 and P8) with a genome size of 41.2 and 44.2 kb pairs, respectively, complete DNA homology, and identical protein composition (main proteins of sizes 39.8, 24.0, 14.8 kilodaltons in sodium dodecyl sulphate-polyacrylamide gel electrophoresis). One phage (a10/J9) with low DNA homology to the other phages was classified into subgroup II. Subgroup III consists of nine phages with a genome size of 33.8 to 36.7 kb pairs and two major structural proteins (30.9 and 24.0 kilodaltons, or 30.9 and 26.3 kilodaltons). In general, phages with different host spectra revealed different restriction enzyme patterns, and DNA homologies of various degrees were detected among all phages tested.

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Authors and Affiliations

1. Institute of Microbiology, Federal Dairy Research Centre, Hermann-Weigmann-Strasse 1, D-2300, Kiel 1, Federal Republic of Germany
   Horst Neve, Uli Krusch & Michael Teuber

Authors

1. Horst Neve
2. Uli Krusch
3. Michael Teuber

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Neve, H., Krusch, U. & Teuber, M. Classification of virulent bacteriophages of Streptococcus salivarius subsp. thermophilus isolated from yoghurt and Swiss-type cheese.Appl Microbiol Biotechnol 30, 624–629 (1989). https://doi.org/10.1007/BF00255370

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• Received: 03 October 1988
• Accepted: 29 November 1988
• Issue date: June 1989
• DOI: https://doi.org/10.1007/BF00255370

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