Genetic and fine structure analysis of unc-26(IV) and adjacent regions in Caenorhabditis elegans (original) (raw)
Summary
The genetic organization of unc-26(IV) and adjacent regions was studied in Caenorhabditis elegans. We constructed a fine structure genetic map of unc-26(IV), a gene that affects locomotion and pharyngeal muscle movement but not muscle structure. Eleven alleles were positioned relative to each other recombinationally and were classified according to phenotypic severity. The unc-26 gene spans at least 0.026 map units, which is exceptionally large for a C. elegans gene. All but one allele, e205, are amorphic alleles. Interestingly, e205 is hypomorphic but also suppressible by the amber suppressor sup-7. Nineteen lethal mutations in the unc-26 region were isolated and characterized. The unc-26 region is subdivided into four zones by five deficiency breakpoints. These mutations fall into 15 complementation groups. The stages of development affected by these mutations were determined.
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- David L. Charest
Present address: Biomedical Research Centre, University of British Columbia, 2222 Health Sciences Mall, V6T 1W5, Vancouver, B.C., Canada - Michael E. Green
Present address: Faculty of Medicine, University of British Columbia, 2194 Health Sciences Mall, V6T 1W5, Vancouver, B.C., Canada
Authors and Affiliations
- Institute of Molecular Biology and Biochemistry, Department of Biological Sciences, Simon Fraser University, V5A 1S6, Burnaby, B.C., Canada
David L. Charest, Denise V. Clark, Michael E. Green & David L. Baillie
Authors
- David L. Charest
You can also search for this author inPubMed Google Scholar - Denise V. Clark
You can also search for this author inPubMed Google Scholar - Michael E. Green
You can also search for this author inPubMed Google Scholar - David L. Baillie
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Communicated by B,H. Judd
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Charest, D.L., Clark, D.V., Green, M.E. et al. Genetic and fine structure analysis of unc-26(IV) and adjacent regions in Caenorhabditis elegans.Mol Gen Genet 221, 459–465 (1990). https://doi.org/10.1007/BF00259412
- Received: 28 February 1990
- Issue Date: May 1990
- DOI: https://doi.org/10.1007/BF00259412