Fermentation products of the anaerobic ciliate Trimyema compressum in monoxenic cultures (original) (raw)

Abstract

A new monoxenic culture of the anaerobic ciliate Trimyema compressum was established on the purple nonsulfur bacterium Rubrivivax gelatinosus. Compared with the first monoxenic culture of T. compressum on a Bacteroides species no significant difference in growth rate was observed. Using both monoxenic cultures the fermentation products of the ciliate, acetate, lactate, formate and succinate were quantified. Ammonium was another product of the ciliates metabolism. The amount of the endproducts was dependent on the amount of bacteria consumed. The ratio of carbon consumed to ammonium formed was 8.7:1 indicating incomplete degradation of nitrogenous compounds of the bacterial biomass. Under microoxic conditions no effect of oxygen on the yield of the ciliate was observed but the amount of acetate and lactate was 25% lower. Cells of T. compressum with Methanobacterium formicicum as deliberately introduced endosymbiont did not form significantly different fermentation products, however, instead of formate methane was detected as product. The yield of the ciliate was not affected. It is concluded that methanogenic bacteria deliberately introduced into the cytoplasm of T. compressum as endosymbionts are pure commensalists.

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1. Fakultät für Biologie, Universität Konstanz, W-7750, Konstanz, Federal Republic of Germany
   S. Holler & N. Pfennig

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1. S. Holler
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2. N. Pfennig
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Holler, S., Pfennig, N. Fermentation products of the anaerobic ciliate Trimyema compressum in monoxenic cultures.Arch. Microbiol. 156, 327–334 (1991). https://doi.org/10.1007/BF00263006

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• Received: 02 May 1991
• Accepted: 25 June 1991
• Issue Date: September 1991
• DOI: https://doi.org/10.1007/BF00263006

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