Heterozygosity and segregation in Candida albicans (original) (raw)
Summary
Strain FC18 of the pathogenic yeast Candida albicans is heterozygous at a gene required for synthesis of methionine and cysteine, and yields auxotrophic derivatives by induced mitotic segregation. Derivatives of FC18 are heterozygous at other genes. Genetic analysis of C. albicans is feasible using induced mitotic recombination.
Access this article
Subscribe and save
- Get 10 units per month
- Download Article/Chapter or eBook
- 1 Unit = 1 Article or 1 Chapter
- Cancel anytime Subscribe now
Buy Now
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Instant access to the full article PDF.
Similar content being viewed by others
References
- Bish JT, Sarachek A (1967) Influences of temperature and adenine concentration upon the cultural instability of a red adenine auxotroph of Candida albicans. Mycologia 59:671–688
Google Scholar - Fink GR (1970) The biochemical genetics of yeast. Meth Enzymol 17A:59–78
Google Scholar - Henson OE, McClary DO (1979) Growth inhibition of Candida albicans by folate pathway inhibitors. Their potential in the selection of auxotrophs. Antonie von Leeuwonhoek. J Microbiol Serol 45:211–223
Google Scholar - Hesse G, Lindner R, Krebs D (1975) Schnelle fluorotometrische deoxyribosespezifische DNS-bestimmung an nicht desintegrierten mikroorganismen mit 3–5 diaminobenzoesäure (DABA). I. Chemisch-analytische Methodik und anwendung auf hefepopulationen. Z Allg Mikrobiol 15:9–18
Google Scholar - Holliday R (1961) Induced mitotic crossing-over in Ustilago maydis. Genet Res Camb 2:231–248
Google Scholar - James AP (1954) Relative frequencies of sectored and non-sectored mutant colonies in yeast as a function of ultraviolet dose. J Bacteriol 67:237–242
Google Scholar - James AP (1955) A genetic analysis of sectoring in ultraviolet-induced variant colonies of yeast. Genetics 40:204–213
Google Scholar - James AP, Lee-Whiting B (1955) Radiation-induced genetic segregation in vegetative cells of diploid yeast. Genetics 40:826–831
Google Scholar - Kwon-Chung KH, Hill WB (1970) Studies on the pink, adenine deficient, strains of Candida albicans. I. Cultural and morphological characteristics. Sabouraudia 8:48–59
Google Scholar - Nakai S, Mortimer RK (1969) Studies of the genetic mechanism of radiation induced mitotic segregation in yeast. Mol Gen Genet 103:329–338
Google Scholar - Olaiya AF, Sogin SJ (1979) Ploidy determination of Candida albicans. J Bacteriol 140:1043–1049
Google Scholar - Pontecorvo G, Roper JA, Forbes E (1953) Genetic recombination without sexual reproduction in Aspergillus niger. J Gen Microbiol 8:198–210
Google Scholar - Pontecorvo G, Sermonti G (1954) Parasexual recombination in Penicillum chrysogenum. J Gen Microbiol 11:94–104
Google Scholar - Sarachek A (1964) Promotion or retardation of the growth of adenine auxotrophs in Candida albicans by purines, pyrimidines, and nucleosides. Antonie von Leeuwonhoek. J Microbiol Serol 30:289–302
Google Scholar - Stern C (1936) Somatic crossing over and segregation in Drosophila melanogaster. Genetics 21:675–730
Google Scholar - Williamson DH, Fennell DJ (1975) The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA. In: Methods in cell biology, XII ed Prescott. Academic Press, New York, p 335
Google Scholar
Author information
Author notes
- William L. Whelan
Present address: Dept. of Zoology Univ. of Iowa Iowa City, 52242, Iowa
Authors and Affiliations
- Department of Microbiology and Public Health, Michigan State University, 48824, East Lansing, Michigan, USA
William L. Whelan, Roger M. Partridge & P. T. Magee
Authors
- William L. Whelan
You can also search for this author inPubMed Google Scholar - Roger M. Partridge
You can also search for this author inPubMed Google Scholar - P. T. Magee
You can also search for this author inPubMed Google Scholar
Additional information
Communicated by G. R. Fink
This is Article No. 9310 of the Michigan Agricultural Experiment Station
Rights and permissions
About this article
Cite this article
Whelan, W.L., Partridge, R.M. & Magee, P.T. Heterozygosity and segregation in Candida albicans.Molec. Gen. Genet. 180, 107–113 (1980). https://doi.org/10.1007/BF00267358
- Received: 27 June 1980
- Issue Date: November 1980
- DOI: https://doi.org/10.1007/BF00267358