prp4 from Schizosaccharomyces pombe, a mutant deficient in pre-mRNA splicing isolated using genes containing artificial introns (original) (raw)

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Summary

We have generated a bank of temperature-sensitive (ts) Schizosaccharomyces pombe mutant strains. About 150 of these mutants were transformed with a ura4 gene containing an artificial intron. We screened these is mutants for mutants deficient in splicing of the ura4 intron. With this approach three mutants were isolated which have a general defect in the splicing process. Two of these mutants fall into the prp1 complementation group and one defines a new complementation group, prp4.

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Authors and Affiliations

1. Department of Bioscience and Biotechnology, Drexel University, 19104, Philadelphia, PA, USA
   George H. Rosenberg, Suresh K. Alahari & Norbert F. Käufer

Authors

1. George H. Rosenberg
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2. Suresh K. Alahari
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3. Norbert F. Käufer
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Communicated by Gajewski

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Rosenberg, G.H., Alahari, S.K. & Käufer, N.F. prp4 from Schizosaccharomyces pombe, a mutant deficient in pre-mRNA splicing isolated using genes containing artificial introns.Mol Gen Genet 226, 305–309 (1991). https://doi.org/10.1007/BF00273617

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• Received: 11 October 1990
• Issue Date: April 1991
• DOI: https://doi.org/10.1007/BF00273617

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