In vivo analysis of chromatin following nystatin-mediated import of active enzymes into Saccharomyces cerevisiae (original) (raw)
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Abstract
In vivo DNA-protein interactions are usually studied at the molecular level using DNA-degrading agents of low molecular weight. In order to be useful, macromolecular probes of chromatin structure, such as enzymes must first cross the cell membrane. In this paper we describe the introduction and evaluation of macromolecules with enzymatic activity into yeast spheroplasts treated with the polyene antibiotic nystatin. We report the low resolution analysis of chromatin structure in the promoter region of the Saccharomyces cerevisiae gene encoding DNA topoisomerase I by this technique using micrococcal nuclease and restriction enzymes.
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- Fondazione “Istituto Pasteur-Fondazione Cenci-Bolognetti” c/o Dipartimento di Genetica e Biologia Molecolare, University di Roma “La Sapienza”, Roma, Italy
Sabrina Venditti & Giorgio Camilloni
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- Sabrina Venditti
You can also search for this author inPubMed Google Scholar - Giorgio Camilloni
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Communicated by C.P. Hollenberg
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Venditti, S., Camilloni, G. In vivo analysis of chromatin following nystatin-mediated import of active enzymes into Saccharomyces cerevisiae.Molec. Gen. Genet. 242, 100–104 (1994). https://doi.org/10.1007/BF00277353
- Received: 23 June 1993
- Issue Date: January 1994
- DOI: https://doi.org/10.1007/BF00277353