The introduction of a transpositionally active copy of retrotransposon GYPSY into the Stable Strain of Drosophila melanogaster causes genetic instability (original) (raw)
Abstract
A previously described genetic system comprising a Mutator Strain (MS) and the Stable Strain (SS) from which it originated is characterized by genetic instability caused by transpositions of the retrotransposon gypsy. A series of genetic crosses was used to obtain three MS derivatives, each containing one MS chromosome (X, 2 or 3) in the environment of SS chromosomes. All derivatives are characterized by elevated frequencies of spontaneous mutations in both sexes. Mutations appear at the premeiotic stage and are unstable. Transformed derivatives of SS and another stable strain 208 were obtained by microinjection of plasmid DNA containing transpositionally active gypsy inserted into the Casper vector. In situ hybridization experiments revealed amplification and active transposition of gypsy in SS derivatives, while the integration of a single copy of gypsy into the genome of 208 does not change the genetic properties of this strain. We propose that genetic instability in the MS system is caused by the combination of two factors: mutation(s) in gene(s) regulating gypsy transposition in SS and its MS derivatives, and the presence of transpositionally active gypsy copies in MS but not SS.
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References
- Bayev AA, Lyubomirskaya NV, Dzhumagaliev EB, Ananiev EV, Amiantova IG, Ilyin YV (1984) Structural organization of transposable element mdg4 from Drosophila melanogaster and nucleotide sequence of its terminal repeats. Nucleic Acids Res 12:3707–3723
Google Scholar - Belyaeva ES, Pasyukova EG, Gvosdev VA, Ilyin YV, Kaidanov LZ (1982) Transpositions of mobile dispersed genes in Drosophila melanogaster and fitness of stocks. Mol Gen Genet 185:324–328
Google Scholar - Bingham PM, Kidwell MG, Rubin GM (1982) The molecular basis of hybrid dysgenesis: the role of the P element, a P specific transposable family. Cell 29:995–1004
Google Scholar - Blackman RK, Gelbart WM (1989) The transposable element hobo of Drosophila melanogaster. In: Berg DE, Howe MM (eds) Mobile DNA. American Society for Microbiology. Washington DC, pp 523–529
Google Scholar - Engels WR (1989) P elements in Drosophila melanogaster. In: Berg DE, Howe MM (eds) Mobile DNA. American Society for Microbiology, Washington DC, pp 437–484
Google Scholar - Finnegan DJ (1989) The I factor and I-R hybrid dysgenesis in Drosophila melanogaster. In: Berg DE, Howe MM (eds) Mobile DNA. American Society for Microbiology, Washington DC, pp 503–507
Google Scholar - Finnegan DJ (1990) Transposable elements. Drosophila Inf Serv 68:371–382
Google Scholar - Gerasimova TI, Mizrokhi LJ, Georgiev GP (1984) Transposition bursts in genetically unstable Drosophila melanogaster. Nature 309:3773–3779
Google Scholar - Ilyin YV, Lyubomirskaya NV, Kim AI (1991) Retrotransposon Gypsy and genetic instability in Drosophila. Genetica 85:13–22
Google Scholar - Karess RE, Rubin GM (1984) Analysis of P transposable element function in Drosophila. Cell 38:135–146
Google Scholar - Kim AI, Belyaeva ES (1991) Transposition of mobile elements gypsy (mdg4) and hobo in germ line and somatic cells of a genetically unstable Mutator strain of Drosophila melanogaster. Mol Gen Genet 229:437–444
Google Scholar - Kim AI, Belyaeva ES, Aslanyan MM (1990) Autonomous transposition of gypsy mobile elements and genetic instability in Drosophila melanogaster. Mol Gen Genet 224:303–308
Google Scholar - Lindsley D, Zimm G (1985) The genome of Drosophila melanogaster. Drosophila Inf Serv 62:1–227
Google Scholar - Lyubomirskaya NV, Arkhipova IR, Ilyin YV (1989) Transcription of Drosophila mobile element mdg4 (gypsy) in heat shocked cells. Genetika (Russia) 26:1720–1728
Google Scholar - Lyubomirskaya NV, Arkhipova IR, Ilyin YV, Kim AI (1990) Molecular analysis of the gypsy (mdg4) retrotransposon in two Drosophila melanogaster strains differing by genetic instability. Mol Gen Genet 223:305–309
Google Scholar - Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Google Scholar - Marlor RL, Parkhurst SM, Corces VG (1986) The Drosophila melanogaster gypsy transposable element encodes putative gene products homologous to retroviral proteins. Mol Cell Biol 6:1129–1134
Google Scholar - Mizrokhi LJ, Obolenkova LA, Priimagi AF, Ilyin YV, Gerasimova TI, Georgiev GP (1985) The nature of unstable insertion mutations and reversions in the locus cut of Drosophila melanogaster: molecular mechanism of transposition memory. EMBO J 4:3781–3787
Google Scholar - Modolell J, Bender W, Meselson MM (1983) Drosophila melanogaster mutations suppressible by suppressor of Hairy-wing are insertions of a 7.3 kilobase mobile element. Proc Natl Acad Sci USA 80:1678–1682
Google Scholar - Pardue ML, Garby SA, Eckhardt RA, Gall JG (1970) Cytological localization of DNA complementary to ribosomal RNA in polytene chromosomes of diptera. Chromosoma 29:268–290
Google Scholar - Peifer M, Bender W (1988) Sequences of the gypsy transposon of Drosophila necessary for its effects on adjacent genes. Proc Natl Acad Sci USA 85:9650–9654
Google Scholar - Pirrotta V (1988) In: Rodriguez RL, Denhardt DT (eds) Vectors: a survey of molecular cloning vectors and their uses. Butterworths, Boston, pp 437–445
Google Scholar - Rubin GM, Spradling AC (1982) Genetic transformation of Drosophila with transposable element vectors. Science 218:348–353
Google Scholar
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Authors and Affiliations
- Dept. of Genetics and Breeding, M.V. Lomonosov Moscow State University, Lenin Hills, 119899, Moscow, Russia
Alexander I. Kim - V.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov St. 32, 117984, Moscow, Russia
Natalia V. Lyubomirskaya, Natalia G. Shostack & Yurii V. Ilyin - Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Sq., 123182, Moscow, Russia
Elena S. Belyaeva
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- Alexander I. Kim
You can also search for this author inPubMed Google Scholar - Natalia V. Lyubomirskaya
You can also search for this author inPubMed Google Scholar - Elena S. Belyaeva
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Communicated by G.P. Georgiev
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Kim, A.I., Lyubomirskaya, N.V., Belyaeva, E.S. et al. The introduction of a transpositionally active copy of retrotransposon GYPSY into the Stable Strain of Drosophila melanogaster causes genetic instability.Molec. Gen. Genet. 242, 472–477 (1994). https://doi.org/10.1007/BF00281799
- Received: 17 March 1993
- Accepted: 18 July 1993
- Issue Date: February 1994
- DOI: https://doi.org/10.1007/BF00281799