The role of subterminal sites of transposable element Ds of Zea mays in excision (original) (raw)
Abstract
Transposition depends on DNA sequences located at or near the termini of the transposon. In the maize transposable element Ds, these sequences were studied by site-directed mutagenesis followed by a transient excision assay in Petunia protoplasts. The transposase-binding AAACGG motifs found in large numbers in the element are important, but none of them is in itself indispensable, for excision. However, mutation of an isolated motif at the 3′ end considerably reduced excisability. The inverted termini were confirmed to be indispensable. Point mutations in regions outside the inverted termini of Ds and not located in the transposase-binding motifs had, in some cases, a pronounced effect on excision frequency. The implications of these findings are discussed.
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- Shivani Chatterjee
Present address: Department of Physiology, McGill University, H3G 1Y6, Montreal, Québec, Canada
Authors and Affiliations
- Institut für Genetik der Universitdt zu Köln, Weyertal 121, D-50931, Germany, Köln
Shivani Chatterjee & Peter Starlinger
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- Shivani Chatterjee
You can also search for this author inPubMed Google Scholar - Peter Starlinger
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Communicated by H. Saedler
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Chatterjee, S., Starlinger, P. The role of subterminal sites of transposable element Ds of Zea mays in excision.Molec. Gen. Genet. 249, 281–288 (1995). https://doi.org/10.1007/BF00290528
- Received: 29 May 1995
- Accepted: 17 July 1995
- Issue Date: May 1995
- DOI: https://doi.org/10.1007/BF00290528