Editing of rps3/rpl16 transcripts creates a premature truncation of the rpl16 open reading frame (original) (raw)

Abstract

Overlapping open reading frames corresponding to maize mitochondrial genes rps3 and rpl16 have been found in Petunia mitochondrial DNA. The DNA region associated with these two genes is part of the Petunia mitochondrial recombination repeat and is iterated three times. Analysis of transcripts from these genes shows that there is RNA editing of the coding regions and that one of the editing sites detected in the open reading frame overlap creates a premature stop codon in the rpl16 sequence. No transcripts were detected that were unedited at this site. Thus, in Petunia editing of rpl16 appears to render this gene nonfunctional.

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Author notes

1. Patricia L. Conklin & Kim D. Pruitt
   Present address: Boyce Thompson Institute for Plant Research at Cornell University, 14853, Ithaca, NY, USA

Authors and Affiliations

1. Section of Genetics and Development, Cornell University, Biotechnology Building, 14853, Ithaca, NY, USA
   Claudia A. Sutton, Patricia L. Conklin, Kim D. Pruitt, A. Jay Calfee, Angela G. Cobb & Maureen R. Hanson

Authors

1. Claudia A. Sutton
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2. Patricia L. Conklin
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3. Kim D. Pruitt
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4. A. Jay Calfee
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5. Angela G. Cobb
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6. Maureen R. Hanson
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Communicated by W.R. Wolstenholme

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Sutton, C.A., Conklin, P.L., Pruitt, K.D. et al. Editing of rps3/rpl16 transcripts creates a premature truncation of the rpl16 open reading frame.Curr Genet 23, 472–476 (1993). https://doi.org/10.1007/BF00312637

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• Received: 14 July 1992
• Accepted: 14 December 1992
• Issue Date: September 1993
• DOI: https://doi.org/10.1007/BF00312637

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