Failure to detect an antimutator phenotype following disruption of the Saccharomyces cerevisiae DDR48 gene (original) (raw)

Abstract

The antimutator phenotype, reportedly conferred by disruption of the Saccharomyces cerevisiae DDR48 gene, was suggested to affect only a specific spontaneous mutational pathway. We attempted to identify the types of mutation that are _DDR48_-dependent by determining the specificity of the ddr48 antimutator. However, disruption of DDR48 did not decrease the rates of spontaneous forward mutation in a plasmid-borne copy of the yeast _SUP4_-o gene, the reversion or suppression of the lys2–1 allele, or forward mutation at the CAN1 locus. Interestingly, the latter gene had been reported previously to be subject to the antimutator effect. DNA sequence analysis of spontaneous _SUP4_-o mutations arising in DDR48 and ddr48 backgrounds provided no evidence for a reduction in the rates of individual mutational classes. Thus, we were unable to verify that disruption of DDR48 causes an antimutator phenotype.

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1. H. Roche
   Present address: Department of Botany and Microbiology, Auburn University, 36801, Auburn, AL, USA

Authors and Affiliations

1. Department of Microbiology, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada
   H. Roche, K. Ramachandran & B. A. Kunz

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1. H. Roche
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2. K. Ramachandran
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3. B. A. Kunz
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Roche, H., Ramachandran, K. & Kunz, B.A. Failure to detect an antimutator phenotype following disruption of the Saccharomyces cerevisiae DDR48 gene.Curr Genet 27, 496–500 (1995). https://doi.org/10.1007/BF00314438

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• Received: 01 September 1994
• Accepted: 03 November 1994
• Issue Date: May 1995
• DOI: https://doi.org/10.1007/BF00314438

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