Plasmid and chromosomal mutants in the iron(III)-aerobactin transport system of Escherichia coli. Use of streptonigrin for selection (original) (raw)

Summary

Assimilation of iron via aerobactin by Escherichia coli requires functions encoded by certain ColV plasmids and by the chromosomal fhu region. Mutants obtained by insertion of the transposons Mu d1 (Ap lac) and Tn_5_ into ColV-K311 revealed that the aerobactin genes are transcribed from the locus that determines aerobactin synthesis to the gene encoding the outer membrane receptor protein for ferric aerobactin uptake. The antibiotic streptonigrin was used for selection of mutants impaired in iron assimilation. A simple assay was devised to identify mutants devoid of aerobactin synthesis. In addition, we performed complementation studies between fhu mutants and fhu genes cloned into pBR322. The genes fhuC,D,B were required for uptake of ferric aerobactin. Mutations in the fhuA gene usually decreased expression of the subsequent fhuC,D,B genes to such an extent that growth on ferric aerobactin as sole iron source was abolished. The results suggest that the genes on the chromosome and on the ColV plasmid are organized in two operons.

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Authors and Affiliations

1. Lehrstuhl Mikrobiologie II der Universität, Auf der Morgenstelle 28, D-7400, Tübingen, Federal Republic of Germany
   Volkmar Braun, Roy Gross, Wolfgang Köster & Luitgart Zimmermann

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1. Volkmar Braun
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2. Roy Gross
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3. Wolfgang Köster
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4. Luitgart Zimmermann
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Communicated by A. Böck

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Braun, V., Gross, R., Köster, W. et al. Plasmid and chromosomal mutants in the iron(III)-aerobactin transport system of Escherichia coli. Use of streptonigrin for selection.Molec Gen Genet 192, 131–139 (1983). https://doi.org/10.1007/BF00327658

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• Received: 18 May 1983
• Issue Date: October 1983
• DOI: https://doi.org/10.1007/BF00327658

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