Kinetochore microtubules and chromosome movement during prometaphase in Drosophila melanogaster spermatocytes studied in life and with the electron microscope (original) (raw)

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Abstract

Prometaphase I chromosome behavior was examined in wild-type Drosophila melanogaster primary spermatocytes. Cine analysis of live cells reveals that bivalents exhibit complex motions that include (1) transient bipolar orientations, (2) simultaneous reorientation of homologous kinetochores, (3) movements not parallel to the spindle axis, and (4) movement along the nuclear membrane. — Kinetochores and kinetochore microtubule have been analyzed for bivalents previously studied in life. The results suggest that most chromosome motions (complex though they may be) can be explained by poleward forces acting on or through kinetochore microtubules that span the distance between the kinetochore and the vicinity of a pole. The results also suggest that the majority of short kinetochore microtubules may be remnants of previous microtubule-mediated associations between a kinetochore and a pole.

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References

• Ault JG, Lin H-PP (1984) Bivalent behavior in Drosophila melanogaster males containing the In(1)sc4Lsc8R X chromosome. Chromosoma 90:222–228
  Google Scholar
• Bajer A (1958) Cine'-micrographic studies on mitosis in endosperm. V. Formation of the metaphase plate. Exp Cell Res 15:370–383
  Google Scholar
• Bauer H, Dietz R, Robbelen C (1961) Die Spermatocytenteilungen der Tipuliden. III. Das Bewegungsverhalten der Chromosomen in Translokationsheterozygoten von Tipula oleracea. Chromosoma 112:116–189
  Google Scholar
• Church K, Lin H-PP (1982) Meiosis in Drosophila melanogaster. II. The prometaphase I kinetochore microtubule bundle and kinetochore orientation in males. J Cell Biol 93:365–373
  Google Scholar
• Dietz R (1956) Die Spermatoytenteilungen der Tipuliden. II. Graphische Analyse der Chromosomenbewegung während der Prometaphase I im Leben. Chromosoma 8:183–211
  Google Scholar
• Granholm NA (1970) Studies on the behavior of bivalents in spermatocytes of Drosophila melanogaster: Electron microscope and in vitro (time-lapse) observations. PhD Thesis, Univ of Oregon
• Goldstein LSB (1981) Kinetochore structure and its role in chromosome orientation during the meiotic division in male Drosophila melanogaster. Cell 25:591–602
  Google Scholar
• Henderson SA, Nicklas RB, Koch CA (1970) Temperature-induced orientation instability during meiosis: An experimental analysis. J Cell Sci 6:323–350
  Google Scholar
• Lin H-PP, Church K (1982) Meiosis in Drosophila melanogaster. III. The effect of orientation disruptor (ord) on gonial mitotic and the meiotic divisions in males. Genetics 102:751–770
  Google Scholar
• Lin H-PP, Ault JG, Church K (1981) Meiosis in Drosophila melanogaster. I. Chromosome identification and kinetochore microtubule numbers during the first and second meiotic divisions in males. Chromosoma 83:507–521
  Google Scholar
• Moens PB, Moens T (1981) Computer measurements and graphics of three-dimensional cellular ultrastructure. J Ultrastruct Res 75:131–141
  Google Scholar
• Nicklas RB (1971) Mitosis. Adv Cell Biol 2:225–297
  Google Scholar
• Nicklas RB (1975) Models, experiments, and mitosis. In: Inoue S, Stevens RE (eds) Molecules and cell movement. Raven, New York, pp 97–117
  Google Scholar
• Nicklas RB, Staehly CA (1967) Chromosome micromanipulation. I. The mechanics of chromosome attachment to the spindle. Chromosoma 49:407–455
  Google Scholar
• Nicklas RB, Brinkley BR, Pepper DA, Kubai DF (1979) Electron microscopy of spermatocytes previously studied in life: Methods and some observations on micromanipulated chromosomes. J Cell Sci 35:87–104
  Google Scholar
• Nicklas RB, Kubai DF, Hays TS (1982) Spindle microtubules and their mechanical associations after micromanipulation in anaphase. J Cell Biol 95:91–104
  Google Scholar
• Rickards GK (1975) Prophase chromosome movements in living house cricket spermatocytes and their relationship to prometaphase, anaphase, and granule movements. Chromosoma 49:407–455
  Google Scholar
• Tates AD (1971) Cytodifferentiation during spermatogenesis in Drosophila melanogaster: An electron microscope study. JH Pasmans, S'Gravenhage
  Google Scholar
• Tippit DH, Pickett-Heaps JD, Leslie R (1980) Cell division in two large pennate diatoms Hantzschia and Nitzschis. III. A new proposal for kinetochore function during prometaphase. J Cell Biol 86:402–416
  Google Scholar

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Authors and Affiliations

1. Department of Zoology, Arizona State University, 85287, Tempe, AZ, USA
   Kathleen Church & Hsiu -Ping Pearl Lin

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1. Kathleen Church
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2. Hsiu -Ping Pearl Lin
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Church, K., Lin, H.P.P. Kinetochore microtubules and chromosome movement during prometaphase in Drosophila melanogaster spermatocytes studied in life and with the electron microscope.Chromosoma 92, 273–282 (1985). https://doi.org/10.1007/BF00329810

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• Received: 29 January 1985
• Revised: 05 March 1985
• Issue Date: August 1985
• DOI: https://doi.org/10.1007/BF00329810

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