Nucleotide sequence of the ends of the conjugative shuttle transposon Tn1545 (original) (raw)
Summary
The conjugative shuttle transposon Tn_1545_ from Streptococcus pneumoniae transposes in various gram-positive bacterial genera following self-transfer and in Escherichia coli after cloning. Analysis of the junction fragments and of the targets before insertion and after excision of the element by DNA hybridization and sequencing indicated that Tn_1545_ (1) is not flanked by terminal repeated sequences in either direct or opposite orientation, (2) is flanked, in an asymmetric fashion, by terminal variable base pairs, one at the left and three at the right of the element, (3) inserts in a target DNA consensus sequence, (4) does not generate duplication of the target DNA upon insertion, and (5) excises precisely.
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References
- Benton WD, Davis RW (1977) Screening λ gt recombinant clones by hybridization to single plaques in situ. Science 196:180
Google Scholar - Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Google Scholar - Borderon E, Bieth G, Horodniceanu T (1982) Genetic and physical studies of Streptococcus faecalis hemolysin plasmids. FEMS Microbiol Lett 14:51–55
Google Scholar - Boyer HW, Roulland-Dussoix D (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41:459–472
Google Scholar - Caillaud F, Carlier C, Courvalin P (1987) Physical analysis of the conuugative shuttle transposon Tn_1545_. Plasmid 17:58–60
Google Scholar - Courvalin P, Carlier C (1986) Transposable multiple antibiotic resistance in Streptococcus pneumoniae. Mol Gen Genet 205:291–297
Google Scholar - Courvalin P, Carlier C (1987) Tn_1545_: a conjugative shuttle transposon. Mol Gen Genet 206:259–264
Google Scholar - Courvalin P, Carlier C, Collatz E (1980) Plamid-mediated resistance to aminocyclitol antibiotics in group D streptococci. J Bacteriol 143:541–551
Google Scholar - Covarrubias L, Bolivar F (1982) Construction and characterisation of new cloning vehicles. VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482 base-pair inverted duplication. Gene 17:79–89
Google Scholar - Di Nocera P, Digan M (1983) A family of oligo-adenylate-terminated transposable sequences in Drosophila melanogaster. J Mol Biol 168:715–727
Google Scholar - Grindley N, Reed R (1985) Transpositional recombination in prokaryotes. Annu Rev Biochem 54:863–896
Google Scholar - Kahman R, Kamp D (1979) Nucleotide sequence of the attachment sites of bacteriophage Mu DNA. Nature 280:247–250
Google Scholar - Koo H-S, Wu H-M, Crothers DM (1986) DNA bending at adenine-thymine tracts. Nature 320:501–506
Google Scholar - Labigne-Roussel A, Witchitz J, Courvalin P (1982) Modular evolution of disseminated Inc7-M plasmids encoding gentamicin resistance. Plasmid 8:215–231
Google Scholar - Lam S, Roth JR (1986) Structural and functional studies of insertion element IS_200_. J Mol Biol 187:157–167
Google Scholar - Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Google Scholar - Messing J (1979) A multipurpose cloning system based on the single-stranded bacteriophage M13. Recombinant DNA Tech Bull 1:43–44
Google Scholar - Murphy E, Löfdahl S (1984) Transposition of Tn_554_ does not generate a target duplication. Nature 307:292–294
Google Scholar - Normore WM (1976) Guanine plus cytosine (GC) composition of the DNA of bacteria, fungi, algae and protozoa. In: Fasman GD (ed) Handbook of biochemistry and molecular biology, vol II. CRC Press, Cleveland, Ohio, pp 65–240
Google Scholar - Novick RP, Clowes RC, Cohen SN, Curtiss III R, Datta N, Falkow S (1976) Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev 40:168–189
Google Scholar - Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Google Scholar - Schachman HK, Adler J, Radding CM, Lehman IR, Kornberg A (1960) Enzymatic synthesis of deoxyribonucleic acid. J Biol Chem 235:3242–3249
Google Scholar - Schnabel HP, Palm P, Dick K, Gramp B (1984) Sequence analysis of the insertion element ISH_1.8_ and of associated structural changes in the genome of phage ϕ H of the archaebacterium Halobacterium halobium. EMBO J 3:1717–1722
Google Scholar - Shapiro JA, ed (1983) In: Mobile genetic elements. Academic Press, New York
Google Scholar - Shinnick TM, Lund E, Smithies O, Blattner FR (1975) Hybridization of labeled RNA to DNA in agarose gels. Nucleic Acids Res 2:1911–1929
Google Scholar - Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Google Scholar - Staden R (1980) A new computer method for storage and manipulation of DNA gel reading data. Nucleic Acids Res 8:3673–3694
Google Scholar
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Author notes
- Frédéric Caillaud
Present address: Genetica, 160 quai de Polangis, F-94340, Joinville-le-pont, France
Authors and Affiliations
- Unité des Agents Antibactériens, CNRS U.A. 271, Institut Pasteur, 28, rue du Dr Roux, F-75724, Paris Cedex 15, France
Frédéric Caillaud & Patrice Courvalin
Authors
- Frédéric Caillaud
- Patrice Courvalin
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Communicated by P. Tiollais
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Caillaud, F., Courvalin, P. Nucleotide sequence of the ends of the conjugative shuttle transposon Tn_1545_.Mol Gen Genet 209, 110–115 (1987). https://doi.org/10.1007/BF00329844
- Received: 29 October 1986
- Issue date: August 1987
- DOI: https://doi.org/10.1007/BF00329844