A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance (original) (raw)

Summary

Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3 + gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3 − mutants grow normally. This selection, based on the loss of orotidine-5′-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.

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Authors and Affiliations

1. Massachusetts Institute of Technology and Whitehead Institute for Biomedical Research, 02142, Cambridge, Massachusetts, USA
   Jef D. Boeke & Gerald R. Fink
2. Laboratoire de Genetique Physiologique, Strasbourg, France
   Francois La Croute

Authors

1. Jef D. Boeke
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2. Francois La Croute
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3. Gerald R. Fink
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Communicated by H. Saedler

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Boeke, J.D., La Croute, F. & Fink, G.R. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.Mol Gen Genet 197, 345–346 (1984). https://doi.org/10.1007/BF00330984

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• Received: 02 August 1984
• Issue Date: November 1984
• DOI: https://doi.org/10.1007/BF00330984

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