Amelioration of the ultraviolet sensitivity of an Escherichia coli recA mutant in the dark by photoreactivating enzyme (original) (raw)

Summary

When a recA strain of Escherichia coli is transformed with a multicopy plasmid, pKY1, carrying the phr gene of E. coli, its extreme ultraviolet sensitivity is decreased. Derivatives of pKY1 were prepared in which the phr gene was inactivated by inserting transposon Tn1000. None of the 20 ph - derivatives decreased the UV sensitivity of the recA strain. In an analogous experiment, we obtained 11 derivatives which failed to decrease the UV sensitivity of the recA strain. None of them complemented phr strain. Furthermore, Tn1000 insertion sites in both types of derivatives were mapped in the same region of the plasmid. From these observations, we propose that the E. coli phr gene product has repair activity in the dark.

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Authors and Affiliations

1. Department of Radiation Biophysics, Kobe University School of Medicine, 650, Kobe, Japan
   Kazuo Yamamoto & Yoshisada Fujiwara
2. Department of Biology, Kobe University Faculty of Science, 657, Kobe, Japan
   Mitsunobu Satake
3. Department of Experimental Chemotherapy, Research Institute for Microbial Diseases, Osaka University, 565, Suita, Japan
   Hideo Shinagawa

Authors

1. Kazuo Yamamoto
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2. Mitsunobu Satake
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3. Hideo Shinagawa
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4. Yoshisada Fujiwara
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Communicated by P.T. Emmerson

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Yamamoto, K., Satake, M., Shinagawa, H. et al. Amelioration of the ultraviolet sensitivity of an Escherichia coli recA mutant in the dark by photoreactivating enzyme.Mol Gen Genet 190, 511–515 (1983). https://doi.org/10.1007/BF00331084

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• Received: 04 January 1983
• Issue Date: June 1983
• DOI: https://doi.org/10.1007/BF00331084

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