Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation (original) (raw)

Summary

A gene bank of partial _Sau_3A restriction fragments of S. pombe DNA has been constructed in the plasmid vector, pDB248′, which is capable of high frequency transformation of S. pombe. Procedures are described which enable plasmids to be recovered from S. pombe by their reintroduction into E. coli. These methods have been used to detect the S. pombe genes lys 1+, ade 6+ and his 2+ in the gene bank by complementation of mutant gene functions, and to physically isolate the lys 1+ gene.

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References

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Authors and Affiliations

1. School of Biology, University of Sussex, BN1 9QG, Brighton, United Kingdom
   David Beach, Melanie Piper & Paul Nurse

Authors

1. David Beach
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2. Melanie Piper
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3. Paul Nurse
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Additional information

Communicated by Ch. Auerbach

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Beach, D., Piper, M. & Nurse, P. Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation.Molec Gen Genet 187, 326–329 (1982). https://doi.org/10.1007/BF00331138

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• Received: 24 May 1982
• Issue Date: October 1982
• DOI: https://doi.org/10.1007/BF00331138

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