The packaging initiation site of phage P22 (original) (raw)

Summary

P22 lysates were grown on Salmonella strains carrying P22 prophages deleted to various extents. Transducing bacterial markers at both sides of the prophage insertion site it could be shown that: (i) transduction of markers can be enhanced by the prophage pac site; (ii) the recognition signal pac is in the area of gene 3 on the phage genome and thus close to the cutting site(s); (iii) transposon Tn10 may also act as a signal for packaging initiation; (iv) (at least) Tn10 initiates packaging sequences in both directions.

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References

• Campbell AM (1962) Episomes. Adv Genet 11:101–145
  Google Scholar
• Casjens S, Huang WM (1982) Initiation of sequential packaging of bacteriophage P22 DNA. J Mol Biol 157:287–298
  Google Scholar
• Chan RK, Botstein D (1972) Genetics of bacteriophage P22. I. Isolation of prophage deletions which affect immunity to super-infection. Virology 49:257–267
  Google Scholar
• Chan RK, Botstein D (1976) Specialized transduction by bacteriophage P22 in Salmonella typhimurium: Genetic and physical structure of the transducing genomes and the prophage attachment site. Genetics 83:433–458
  Google Scholar
• Chelala CA, Margolin P (1974) Effects of deletions of cotransduction linkage in Salmonella typhimurium: Evidence that bacterial chromosome deletions affect the formation of transducing DNA fragments. Mol Gen Genet 131:97–112
  Google Scholar
• Chelala CA, Margolin P (1976) Evidence that HT mutant strains of bacteriophage P22 retain an altered form of substrate specificity in the formation of transducing particles in Salmonella typhimurium. Genet Res 27:315–322
  Google Scholar
• Deans RJ, Jackson EN (1979) Restriction endonuclease _Hind_III cleavage site map of bacteriophage P22. Virology 95:359–372
  Google Scholar
• Jackson EN, Jackson DA, Deans RJ (1978) _Eco_RI analysis of bacteriophage P22 DNA packaging. J Mol Biol 118:365–388
  Google Scholar
• Krayewska-Grynkiewicz K, Kŀopotowski T (1979) Altered linkage values in phage P22-mediated transduction caused by distant deletions or insertions in donor chromosomes. Mol Gen Genet 176:87–93
  Google Scholar
• Kwoh DY, Kemper J (1978a) Bacteriophage P22-mediated specialized transduction in Salmonella typhimurium: High frequency of aberrant prophage excision. J Virol 27:519–534
  Google Scholar
• Kwoh DY, Kemper J (1978b) Bacteriophage P22-mediated specialized transduction in Salmonella typhimurium: Identification of different types of specialized transducing particles. J Virol 27:535–550
  Google Scholar
• Ozeki H (1959) Chromosome fragments participating in transduction in Salmonella typhimurium. Genetics 44:457–470
  Google Scholar
• Raj AS, Raj AY, Schmieger H (1974) Phage genes involved in the formation of generalized transducing particles in Salmonella phage P22. Mol Gen Genet 135:175–184
  Google Scholar
• Sanderson KE, Hartman PE (1978) Linkage map of Salmonella typhimurium. Edition V. Microbiol Rev 42:471–519
  Google Scholar
• Schmieger H (1968) Die molekulare Struktur transduzierender Partikel beim _Salmonella_-Phagen P22. I. Dichtegradienten-Untersuchungen an intakten Phagen. Mol Gen Genet 102:336–347
  Google Scholar
• Schmieger H (1972) Phage P22-mutants with increased or decreased transduction abilities. Mol Gen Genet 119:75–88
  Google Scholar
• Schmieger H, Backhaus H (1976) Altered cotransduction frequencies exhibited by HT-mutants of _Salmonella_-phage P22. Mol Gen Genet 143:307–309
  Google Scholar
• Smith HO (1968) Defective phage formation by lysogens of integration deficient phage P22 mutants. Virology 34:203–223
  Google Scholar
• Smith HO, Levine M (1967) A phage P22 gene controlling integration of prophage. Virology 31:207–216
  Google Scholar
• Tye BK (1976) A mutant of phage P22 with randomly permuted DNA. J Mol Biol 100:421–426
  Google Scholar
• Tye BK, Huberman JA, Botstein D (1974) Non-random circular permutation of phage P22 DNA. J Mol Biol 85:501–532
  Google Scholar
• Weaver S, Levine M (1978) Replication in situ and DNA encapsulation following induction of an excision-defective lysogen of Salmonella bacteriophage P22. J Mol Biol 118:389–411
  Google Scholar

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Authors and Affiliations

1. Institut für Genetik und Mikrobiologie der Universität München, Maria-Ward-Strasse 1 a, D-8000, München 19, Federal Republic of Germany
   Barbara Kufer, Horst Backhaus & Horst Schmieger

Authors

1. Barbara Kufer
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2. Horst Backhaus
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3. Horst Schmieger
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Communicated by H. Saedler

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Kufer, B., Backhaus, H. & Schmieger, H. The packaging initiation site of phage P22.Molec Gen Genet 187, 510–515 (1982). https://doi.org/10.1007/BF00332636

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• Received: 17 July 1982
• Issue Date: October 1982
• DOI: https://doi.org/10.1007/BF00332636

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