Gene conversion in nonsense suppressors of Schizosaccharomyces pombe (original) (raw)

Summary

The frequency of gene conversion was assessed at the anticodon site of the sup3 gene of Schizosaccharomyces pombe. In order to detect a possible influence of the genetic background on the relative frequency of post-meiotic segregations amongst conversions, three similar crosses were analyzed which differed as follows: In cross I the two parents were derived by spontaneous mutation from one and the same strain. The heterogeneity of the genetic background between these two parent strains is assumed to be at a minimum level. In cross II the crossing partners were strains of the Bernese stock collection and differ probably in their genetic background to some extent. Last, in cross III, one of the parent strains was ten times repeatedly mutagenized with nitrosoguanidine in order to introduce cryptic mutations in the genome. A maximum degree of background heterogeneity between parents is expected for this cross. Neither the total conversion frequency nor the frequency of post-meiotic segregations amongst conversions were found to be significantly different in these three crosses.

In addition, no effect of the radiation-sensitive mutation, rad2-44, and of two mutator mutations, mut1-4 and mut2-9, on the conversion pattern could be detected.

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References

• Cavalli-Sforza, L.: Biometrie. Grundzüge biologisch-medizinischer Statistik. Stuttgart: Gustav Fischer 1972
  Google Scholar
• Dicaprio, L., Hastings, P.J.: Post-meiotic segregation in strains of S. cerevisiae unable to excise pyrimidine dimers. Mutat. Res. 37, 137–140 (1976)
  Google Scholar
• Fincham, J.R.S., Holliday, R.: An explanation of fine structure map expansion in terms of excision repair. Mol. Gen. Genet. 109, 309–322 (1970)
  Google Scholar
• Fogel, S., Mortimer, R.K.: Gene conversion and mismatched base repair in hybrid DNA. Genetics 77, s22 (1974)
• Gutz, H., Doe, F.J.: Two different h - mating types in Schizosaccharomyces pombe. Genetics 74, 563–569 (1973)
  Google Scholar
• Gutz, H., Heslot, H., Leupold, U., Loprieno, N.: Schizosaccharomyces pombe. In: Handbook of genetics (R.C. King, ed.), vol. 1, pp. 395–446. New York: Plenum Press 1974
  Google Scholar
• Hawthorne, D.C., Leupold, U.: Suppressor mutations in yeast. Curr. Top. Microbiol. Immunol. 64, 1–47 (1974)
  Google Scholar
• Holiday, R.: A mechanism for gene conversion in fungi. Genet. Res. (Camb) 5, 282–304 (1964)
  Google Scholar
• Holliday, R., Dickson, J.M.: The detection of post-meiotic segregation without tetrad analysis in Ustilago maydis. Mol. Gen. Genet.153, 331–335 (1977)
  Google Scholar
• Kohli, J., Hottinger, H., Munz, P., Strauss, A., Thuriaux, P.: Genetic mapping in Schizosaccharomyces pombe by mitotic and meiotic analysis and induced haploidization. Genetics 87, 471–489 (1977)
  Google Scholar
• Kohli, J., Kwong, T., Altruda, F., Söll, D., Wahl, G.: Characterization of a UGA-suppressing serine tRNA from Schizosaccharomyces pombe with the help of a new in vitro assay system for eukaryotic suppressor tRNAs. (1978, in press)
• Meselson, M.S., Radding, C.M.: A general model for genetic recombination. Proc. Natl. Acad. Sci. U.S.A. 72, 358–361 (1975)
  Google Scholar
• Munz, P.: On some properties of five mutator alleles in Schizosaccharomyces pombe. Mutat. Res. 29, 155–157 (1975)
  Google Scholar
• Nevers, P., Spatz, H. -C.: Escherichia coli mutants uvrD and uvrE deficient in gene conversion of λ heteroduplexes. Mol. Gen. Genet. 139, 233–243 (1975)
  Google Scholar
• Paquette, N., Rossignol, J. -L.: Gene conversion spectrum of 15 mutants giving post-meiotic segregation in the b2 locus of Ascobolus immersus. Mol. Gen. Genet. 163, 313–326 (1978)
  Google Scholar
• Schüpbach, M.: The isolation and genetic classification of UV-sensitive mutants of Schizosaccharomyces pombe. Mutat. Res. 11, 361–371 (1971)
  Google Scholar

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Authors and Affiliations

1. Institute of General Microbiology, University of Bern, Altenbergrain 21, CH-3013, Bern, Switzerland
   P. Munz & U. Leupold

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1. P. Munz
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2. U. Leupold
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Communicated by F. Kaudewitz

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Munz, P., Leupold, U. Gene conversion in nonsense suppressors of Schizosaccharomyces pombe.Molec. gen. Genet. 170, 145–148 (1979). https://doi.org/10.1007/BF00337789

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• Received: 16 October 1978
• Issue Date: January 1979
• DOI: https://doi.org/10.1007/BF00337789

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