Isolation and genetic characterization of a thymineless death-resistant mutant of Escherichia coli K12: Identification of a new mutation (recQ1) that blocks the RecF recombination pathway (original) (raw)

Summary

An Escherichia coli K12 mutant resistant to thymineless death (TLD) was isolated, and its genetic analysis led us to identify a new mutation (recQ1) located between corA and metE on the standard linkage map. The mutation was found to result in increased sensitivity to ultraviolet light and deficiency in conjugational recombination when placed in the recBC sbcB background, indicating that it blocked the RecF pathway of recobbination. It seemed likely that this mutation is also capable of causing partial resistance to TLD, but we reserve the possibility of a separate mutation closely linked to recQ1 giving rise to this phenotype. The original mutant was shown to carry an additional mutation probably in the vicinity of the uhp locus, which was also required for the full TLD resistance of the mutant to be expressed.

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Abbreviations

UV:

ultraviolet light

NG:

N-methyl-N'-nitro-N-nitrosoguanidine

Km:

Kanamycin

Sm:

streptomycin

TLD:

thymineless death

1 :

resistant

s :

sensitive

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Author notes

1. Yasuko Nakayama
   Present address: Medical College of Miyazaki, Miyazaki, Japan

Authors and Affiliations

1. Department of Microbiology, School of Dentistry, Kyushu University, 812, Fukuoka, Japan
   Hiroaki Nakayama, Koji Nakayama, Ritsuko Nakayama, Nobuto Irino & Yasuko Nakayama
2. Department of Biological Sciences, Stanford University, 94305, Stanford, California, USA
   Philip C. Hanawalt

Authors

1. Hiroaki Nakayama
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2. Koji Nakayama
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3. Ritsuko Nakayama
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4. Nobuto Irino
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5. Yasuko Nakayama
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6. Philip C. Hanawalt
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Communicated by P.T. Emmerson

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Nakayama, H., Nakayama, K., Nakayama, R. et al. Isolation and genetic characterization of a thymineless death-resistant mutant of Escherichia coli K12: Identification of a new mutation (recQ1) that blocks the RecF recombination pathway.Mol Gen Genet 195, 474–480 (1984). https://doi.org/10.1007/BF00341449

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• Received: 24 February 1984
• Issue Date: July 1984
• DOI: https://doi.org/10.1007/BF00341449

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