Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn10 (original) (raw)

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Summary

We have cloned the tolC gene of E. coli K-12 into pSF2124 by using transposon Tn_10_ as the marker to first isolate the relevant DNA fragment. The gene is on a 10.5 kb Eco_RI fragment, and Tn_5 insertion mutagenesis locates the gene near one end of this _Eco_RI fragment. An _Eco_RI-_Pst_I fragment has been subcloned into pBR322 to facilitate further analysis of the gene.

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Abbreviations

Tris:

Tris (hydroxymethyl) aminomethane

EDTA:

Ethylenediamine tetra-acetic acid

DOC:

Sodium deoxycholate

DNA:

Deoxyribonucleic acid

SDS:

Sodium dodecyl sulphate

kb:

kilo base pairs

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Authors and Affiliations

1. Department of Microbiology and Immunology, The University of Adelaide, 5000, Adelaide, South Australia
   Renato Morona & Peter Reeves

Authors

1. Renato Morona
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2. Peter Reeves
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Communicated by D. Sherratt

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Morona, R., Reeves, P. Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn_10_.Mol Gen Genet 184, 430–433 (1981). https://doi.org/10.1007/BF00352517

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• Received: 10 October 1981
• Issue Date: December 1981
• DOI: https://doi.org/10.1007/BF00352517

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