Mismatch-stimulated plasmid integration in yeast (original) (raw)

Summary

A single base pair mismatch (G:T or A:C) in the CYC1 gene of the integrative plasmid pAB218 stimulates up to a five-fold integration into the yeast chromosome. Analysis of chromosomal sites of plasmid integration suggests that the mismatch-stimulated integration is not targeted as would be expected if crossovers, localised in the region of the mismatch, were a necessary step in mismatch repair. Instead, the observed mismatch-stimulated plasmid integration could be due to potentially recombinogenic structures formed during mismatch repair, such as single-stranded gaps or denatured DNA regions extending around the plasmid molecule.

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Author notes

1. Zoran Zgaga
   Present address: Faculty of Food and Biotechnology, Laboratory of Biology and Microbial Genetics, University of Zagreb, Kršnjavoga 25, YU-4100, Zagreb, Yugoslavia

Authors and Affiliations

1. Institut Curie-Biologie, Centre Universitaire, Bât. 110, F-91405, Orsay, France
   Zoran Zgaga, Roland Chanet & Francis Fabre
2. Institut Jacques Monod, Tour 43, 2 Place Jussieu, F-75251, Paris Cédex 05, France
   Miroslav Radman

Authors

1. Zoran Zgaga
2. Roland Chanet
3. Miroslav Radman
4. Francis Fabre

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Communicated by M. S. Esposito

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Zgaga, Z., Chanet, R., Radman, M. et al. Mismatch-stimulated plasmid integration in yeast.Curr Genet 19, 329–332 (1991). https://doi.org/10.1007/BF00355064

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• Received: 30 July 1990
• Accepted: 20 September 1990
• Issue date: April 1991
• DOI: https://doi.org/10.1007/BF00355064

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