Meiotic chromosome condensation and pairing in Saccharomyces cerevisiae studied by chromosome painting (original) (raw)

Abstract

Non-isotopic high resolution in sity hybridization was applied to cytological preparations of sporulating yeast cells. Ribosomal DNA (rDNA) and chromosome V-specific recombinant lambda clones were used to tag individual chromosomes and chromosome subregions. This allowed the study of chromosome behaviour during early meiotic prophase. It was found that chromatin becomes condensed and homologous DNA sequences then appear to become aligned prior to synaptonemal complex formation.

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Author notes

1. Harry Scherthan
   Present address: Erwin-Schroedinger-Strasse, Division of Human Genetics and Human Biology of the University, W-6750, Kaiserslautern, Federal Republic of Germany

Authors and Affiliations

1. Department of Cytology and Genetics, Institute of Botany, University of Vienna, Rennweg 14, A-1030, Vienna, Austria
   Harry Scherthan, Josef Loidl & Dieter Schweizer
2. Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030, Vienna, Austria
   Tillman Schuster

Authors

1. Harry Scherthan
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2. Josef Loidl
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3. Tillman Schuster
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4. Dieter Schweizer
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by E.R. Schmidt

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Scherthan, H., Loidl, J., Schuster, T. et al. Meiotic chromosome condensation and pairing in Saccharomyces cerevisiae studied by chromosome painting.Chromosoma 101, 590–595 (1992). https://doi.org/10.1007/BF00360535

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• Received: 10 June 1992
• Accepted: 29 June 1992
• Issue Date: October 1992
• DOI: https://doi.org/10.1007/BF00360535

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