Skin-homing T lymphocytes: detection of cutaneous lymphocyteassociated antigen (CLA) by HECA-452 in normal human skin (original) (raw)

Abstract

The immigration of circulating T cells into specific tissues is directed by the interaction between adhesion molecules on lymphocyte subpopulations and their ligands on vascular endothelium. Of these, endothelial leucocyte adhesion molecule (ELAM-1), weakly expressed in normal human skin (NHS), seems to be the counter-structure for cutaneous lymphocyte-associated antigen (CLA). CLA is a 200 kDa cell-surface glycoprotein of which the sugar moieties sialyl Le(a) and sialyl Le(x) are the possible epitopes recognized by the monoclonal antibody HECA-452. HECA-452 was originally described as a marker for lymphoid organ high endothelial cells, but 16% of peripheral-blood-derived T cells react with this antibody. We studied the expression of CLA on the cellular constituents of the skin immune system (SIS). By applying immunohistochemical double staining, 41% of CD3+ T cells, 44% of CD4+ T cells and 31% of CD8+ T cells were found to express CLA. Keratinocytes, CD1a+ Langerhans cells (LC) and endothelial cells did not express HECA-452 in significant numbers in NHS. Monocytes were found to express HECA-452 in 14% of CD68+ cells. CLA expression was present on a relatively low percentage of T cells and subsets localized distant from NHS vessels, suggesting loss of the molecule during further migration after transendothelial passage. However, intraepidermal T cells expressed CLA in similar percentages to T cells localized directly perivascularly. Our findings support the notion that CLA expression by T cells is associated with their homing into cutaneous structures.

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Authors and Affiliations

  1. Department of Dermatology A0-235, University of Amsterdam, Academisch Medisch Centrum, Meibergdreef 9, NL-1105, AZ Amsterdam, The Netherlands
    J. D. Bos, O. J. de Boer, E. Tibosch & P. K. Das
  2. Department of Pathology, University of Amsterdam, Academisch Medisch Centrum, Amsterdam, The Netherlands
    P. K. Das & S. T. Pals

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  1. J. D. Bos
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  2. O. J. de Boer
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  3. E. Tibosch
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  4. P. K. Das
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  5. S. T. Pals
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Bos, J.D., de Boer, O.J., Tibosch, E. et al. Skin-homing T lymphocytes: detection of cutaneous lymphocyteassociated antigen (CLA) by HECA-452 in normal human skin.Arch Dermatol Res 285, 179–183 (1993). https://doi.org/10.1007/BF00372006

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