Effects of training in normoxia and normobaric hypoxia on human muscle ultrastructure (original) (raw)

Abstract

The adaptive response of skeletal muscle to training in normoxia and in severe normobaric hypoxia was studied. The first group of five male subjects trained for 3 weeks on a bicycle (2 h/day, 6 days/week) in normoxia (Control training, Con T). A second group of five subjects trained in an ambient FIO2 decreasing progressively from 12.7% to a final level of 10.0% (hypoxic training, Hyp T). Fourteen months later, these subjects trained in normoxia at the same absolute power (normoxic training, Nor T). Peak oxygen consumption (\(\dot V\)O2 max) was measured in normoxic and hypoxic conditions. Biopsies from the vastus lateralis muscle were analysed for fibre size, capillary and ultrastructural composition. Nor T had no effect on muscle tissue or \(\dot V\)O2 max. Con T increased volume density of total mitochondria and lipids by 36 and 135% respectively (P<0.05). Hyp T induced a 10% increase (P<0.05) in peak \(\dot V\)O2 max measured in hypoxia. Mean fibre cross-sectional area, interfibrillar mitochondrial volume density and capillary-to-fibre ratio were increased (P<0.05) by 10, 42 and 13% respectively in the Hyp T group. These results suggest that training at the same relative workload in normoxia and hypoxia have similar, but not identical, effects on muscle tissue. If training in normoxia is carried out at the same absolute workload as in severe hypoxia, no significant effects are observed.

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References

  1. Bender PR, Groves BM, McCullough RE, McCullough RG, Huang SY, Hamilton AJ, Wagner PD, Cymerman A, Reeves JT (1988) Oxygen transport to exercising leg in chronic hypoxia. J Appl Physiol 65: 2592–2597
    Google Scholar
  2. Boutellier U, Deriaz O, Diprampero PE, Cerretelli P (1990) V. Aerobic performance at altitude: Effects of acclimatization and hematocrit with reference to training. Int J Sports Med 11 (Supplement 1): S 21-S 26
    Google Scholar
  3. Cerretelli P, Hoppeler H (1993) Morphologic and metabolic response to chronic hypoxia: the muscle system. In: Lahiri S (ed) Handbook of physiology. Adaptation to the environment. American Physiological Society, Bethesda (in press)
    Google Scholar
  4. Elander A, Idstroem JP, Schersten T, Bylund-Fellenius AC (1985) Metabolic adaptation to reduced muscle blood flow. I. Enzyme and metabolite alterations. Am J Physiol 249: E 63-E 69
    Google Scholar
  5. Gaesser GA, Poole DC, Gardner BP (1984) Dissociation between \(\dot V\)O2max and ventilatory threshold responses to endurance training. Eur J Appl Physiol 53: 242–247
    Google Scholar
  6. Green HJ, Sutton JR, Cymerman A, Young PM, Houston CS (1989) Operation Everest II: Adaptations in human skeletal muscle. J Appl Physiol 66: 2454–2461
    Google Scholar
  7. Henriksson KG (1979) Semi-open muscle biopsy technique. A simple outpatient procedure. Acta Neurol Scand 59: 317–323
    Google Scholar
  8. Henriksson K, Reitman JS (1977) Time course of changes in human skeletal muscle succinate dehydrogenase and cytochrome oxydase activities and maximal oxygen uptake with physical activity and inactivity. Acta Physiol Scand 99: 91–97
    Google Scholar
  9. Hickson RC, Bomze HA, Holloszy JO (1977) Linear increase in aerobic power induced by a strenuous program of endurance exercise. J Appl Physiol 42: 372–376
    Google Scholar
  10. Hochachka PW, Stanley C, Merkt J, Sumar-Kalinowski J (1982) Metabolic meaning of elevated levels of oxidative enzymes in high altitude adapted animals: an interpretive hypothesis. Respir Physiol 52: 303–313
    Google Scholar
  11. Hoppeler H, Howald H, Conley KE, Lindstedt SL, Claassen H, Vock P, Weibel ER (1985) Endurance training in humans: aerobic capacity and structure of skeletal muscle. J Appl Physiol 59: 320–327
    Google Scholar
  12. Hoppeler H, Kleinert E, Schlegel C, Claassen H, Howald H, Kayar S, Cerretelli P (1990) II. Morphological adaptations of human skeletal muscle to chronic hypoxia. Int J Sports Med 11 (Supplement 1): S 3-S 9
    Google Scholar
  13. Howald H, Pette D, Simoneau JA, Uber A, Hoppeler H, Cerretelli P (1990) III. Effects of chronic hypoxia on muscle enzyme activities. Int J Sports Med 11 (Supplement 1): S 10-S 14
    Google Scholar
  14. MacDougall JD, Green HJ, Sutton JR, Coates G, Cymerman A, Young P, Houston CS (1991) Operation Everest II: structural adaptations in skeletal muscle in response to extreme simulated altitude. Acta Physiol Scand 142: 421–427
    Google Scholar
  15. Preedy VR, Sugden PH (1989) The effects of fasting or hypoxia on rates of protein synthesis in vivo in subcellular fractions of heart and gastrocnemius muscle. Biochem J 257: 519–527
    Google Scholar
  16. Rösler K, Hoppeler H, Conley KE, Claassen H, Gehr P, Howald H (1985) Transfer effects in endurance exercise. Adaptations in trained and untrained muscles. Eur J Appl Physiol 54: 355–362
    Google Scholar
  17. Terrados N, Jansson E, Sylven C, Kaijser L (1990) Is hypoxia a stimulus for synthesis of oxidative enzymes and myoglobin? J Appl Physiol 68: 2369–2372
    Google Scholar
  18. Weibel ER (1979) Practical methods for biological morphometry. (Stereological methods vol 1) Academic Press, London
    Google Scholar
  19. Wolfel EE, Groves BM, Brooks GA, Butterfield GE, Mazzeo RS, Moore LG, Sutton JR, Bender PR, Dahms TE, McCul-lough RE, McCullough RG, Huang SY, Sun SF, Grover RF, Hultgren HN, Reeves JT (1991) Oxygen transport during steady-state submaximal exercise in chronic hypoxia. J Appl Physiol 70: 1129–1136
    Google Scholar
  20. Yoshida T, Udo M, Ohmori T, Matsumoto Y, Uramoto T, Yamamoto K (1992) Day-to-day changes in oxygen uptake kinetics at the onset of exercise during strenuous endurance training. Eur J Appl Physiol 64: 78–83
    Google Scholar

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Authors and Affiliations

  1. Laboratoire de Physiologie, Faculté de Médecine de Lyon Grange Blanche, URA 1341 CNRS, F-69373, Lyon Cedex 08, France
    D. Desplanches
  2. Anatomisches Institut, Universität Bern, CH-3000, Bern, Switzerland
    H. Hoppeler & H. Claassen
  3. Laboratoire de Physiologie-G. I. P. Exercice, Faculté de Médecine de St.-Etienne, F-42023, St.-Etienne Cedex 2, France
    M. T. Linossier, C. Denis, D. Dormois & A. Geyssant
  4. Centre de Recherche en Biomécanique Articulaire, Faculté de Médecine de Lyon-Sud, F-69921, Oullins, France
    J. R. Lacour

Authors

  1. D. Desplanches
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  2. H. Hoppeler
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  3. M. T. Linossier
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  4. C. Denis
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  5. H. Claassen
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  6. D. Dormois
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  7. J. R. Lacour
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  8. A. Geyssant
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Desplanches, D., Hoppeler, H., Linossier, M.T. et al. Effects of training in normoxia and normobaric hypoxia on human muscle ultrastructure.Pflügers Arch 425, 263–267 (1993). https://doi.org/10.1007/BF00374176

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Key words

Normobaric hypoxia