MAL63 codes for a positive regulator of maltose fermentation in Saccharomyces cerevisiae (original) (raw)

Summary

Genetic analysis of the MAL6 locus has previously yielded mal6 mutants which fall into a single complementation group and which are noninducible for maltase and maltose permease. However, the strains used in these studies contained additional partially functional copies of MAL1 (referred to as MAL1g) and MAL3 (referred to as MAL3g). Using a strain lacking MALg genes, we have isolated two classes of mutants and these classes correspond to mutations in MAL63 and MAL61, two genes of the MAL6 complex. Disruptions of MAL63 are noninducible for maltase and maltose permease and for their corresponding mRNAs. The mal6 mutants are shown to map to MAL63 Inducer exclusion as a cause of the noninducible phenotype of the mal63 mutations has been eliminated by constructing a ma163 mutant in a strain constitutive for maltose permease; the strain remains noninducible. These results rigorously demonstrate that MAL63 is a regulatory gene which plays a positive role in the regulation of maltose fermentation.

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References

• Barnett JA, Sims AP (1982) J Gen Microbiol 128:2302–2312
  Google Scholar
• Chang YSM (1985) Mutational analysis of the MAL6 locus of Saccharomyces. PhD thesis, Wayne State University School of Medicine
• Charron MJC (1987) Structural and functional analysis of the MAL loci of Saccharomyces. PhD thesis, The City University of New York
• Charron MJC, Dubin RA, Michels CA (1986) Mol Cell Bio 6:3891–3899
  Google Scholar
• Cohen JD, Goldenthal MJ, Chow T, Buchferer B, Marmur J (1985) Mol Gen Genet 200:1–8
  Google Scholar
• Douglas HC, Hawthorne DC (1964) Genetics 49:837–844
  Google Scholar
• Douglas HC, Hawthorne DC (1966) Genetics 54:911–916
  Google Scholar
• Dubin R, Needleman RB, Gossett, D, Michels CA (1985) J Bacteriol 164:605–610
  Google Scholar
• Dubin R, Perkins E, Needleman RB, Michels CA (1986) Mol Cell Biol 6:2757–2765
  Google Scholar
• Dubin R, Charron MJ, Haut SR, Needleman RB, Michels CA (1988) Mol Cell Biol 8:1027–1035
  Google Scholar
• Falco SC, Rose M, Botstein D (1983) Genetics 105:843–856
  Google Scholar
• Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168
  Google Scholar
• Michels CA, Needleman RB (1983) Mol Gen Genet 191:225–230
  Google Scholar
• Michels CA, Needleman RB (1984) J Bacteriol 157:949–952
  Google Scholar
• Naumov GI (1971) Comparative genetics in yeast V. Genetika 7:141–148
  Google Scholar
• Needleman RB (1975) The control of maltose fermentation in yeast. PhD thesis, City University of New York
• Needleman RB, Eaton NR (1974) Mol Gen Genet 133:135–140
  Google Scholar
• Needleman RB, Michels CA (1983) Mol Cell Biol 3:796–802
  Google Scholar
• Needleman RB, Kaback DB, Dubin R, Perkins EL, Rosenberg NG, Sutherland KA, Forrest DB, Michels CA (1984) Proc Natl Acad Sci USA 81:2811–2815
  Google Scholar
• Orr-Weaver TT, Szostak JW, Rothstein RJ (1983) Methods Enzymol 101:228–245
  Google Scholar
• Rothstein RJ (1983) Methods Enzymol 101:202–211
  Google Scholar
• Serrano R (1977) Eur J Biochem 80:97–102
  Google Scholar
• Southern EM (1975) J Mol Biol 98:503–517
  Google Scholar
• ten Berge AMA, Zoutewelle G, van de Poll KW (1973a) Mol Gen Genet 123:233–246
  Google Scholar
• ten Berge AMA, Zoutewelle G, van de Poll KW, Bloemers HPJ (1973b) Mol Gen Genet 125:139–146
  Google Scholar
• ten Berge AMA, Zoutewelle G, Needleman RB (1974) Mol Gen Genet 131:113–121
  Google Scholar
• Zimmermann FK, Eaton NR (1974) Mol Gen Genet 134:261–272
  Google Scholar

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Authors and Affiliations

1. Department of Biochemistry, Wayne State University School of Medicine, 48201, Detroit, MI, USA
   Young Sook Change, Edward Perkins, Doug Forrest & Richard B. Needleman
2. Department of Biology, Queens College and Graduate School, The City University of New York, 11367, Flushing, NY, USA
   Robert A. Dubin & Corinne A. Michels

Authors

1. Young Sook Change
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2. Robert A. Dubin
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3. Edward Perkins
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4. Doug Forrest
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5. Corinne A. Michels
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6. Richard B. Needleman
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Change, Y.S., Dubin, R.A., Perkins, E. et al. MAL63 codes for a positive regulator of maltose fermentation in Saccharomyces cerevisiae.Curr Genet 14, 201–209 (1988). https://doi.org/10.1007/BF00376740

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• Issue Date: September 1988
• DOI: https://doi.org/10.1007/BF00376740

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