Individual interphase chromosome domains revealed by in situ hybridization (original) (raw)

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Summary

The position and arrangement of individual chromosomes in interphase nuclei were examined in mouse-human cell hybrids by in situ hybridization of biotinylated human DNA probes. Intense and even labeling of human chromosomes with little background was observed when polyethylene glycol and Tween-20 were included in hybridization solutions. Human interphase chromosomes were separated from each other in the nucleus, and were confined to well localized domains. Hybrid cells with a single human chromosome showed a reproducible position of this chromosome in the nucleus. Some chromosomes appeared to have a characteristic folding pattern in interphase. Optical section as well as electron microscopy of labeled regions revealed the presence of 0.2 μm wide fibers in each interphase domain, as well as adjacent, locally extended 500 nm fibers. Such fibers are consistent with previously proposed structural models of interphase chromosomes.

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Authors and Affiliations

1. Yale University School of Medicine, 06510, New Haven, CT, USA
   Laura Manuelidis

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1. Laura Manuelidis
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Manuelidis, L. Individual interphase chromosome domains revealed by in situ hybridization.Hum Genet 71, 288–293 (1985). https://doi.org/10.1007/BF00388453

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• Received: 02 October 1985
• Issue Date: December 1985
• DOI: https://doi.org/10.1007/BF00388453

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