A comparison of the killer character in different yeasts and its classification (original) (raw)
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Abstract
The interactions between 20 killer yeasts of various genera and species were examined. Ten distinct groups were recognised with respect to killer activity and 10 distinct groups with respect to resistance to killer action. Using both killing and resistance phenotypes, 13 classes of killer yeast were found. With the exception of Torulopsis glabrata NCYC 388, non-Saccharomyces strains of yeast were not killed by a member of the genus Saccharomyces.
The killer character of the 3 killing groups of Saccharomyces identified could be cured by treatment with cycloheximide or incubation at elevated temperature and the effectiveness of these procedures was indicative of the category of killer yeast examined. Killer yeasts not belonging to the genus Saccharomyces could not be cured of their activity. Double-stranded ribonucleic acids were extracted only from Saccharomyces spp. and the molecular weights of the species present were a function of the killer class to which a strain belonged.
By an analysis of the effects of proteolytic enzymes, temperature and pH on killer activity and by gel chromatography of crude preparations of killer factors, the toxins of different killer classes were shown to be biochemically distinct. However all toxins had certain properties in common consistent with there being a protein component essential to killer action.
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References
- Badin, J., Jackson, C. and Schubert, M. 1953. Improved method for determination of plasma polysaccharides with tryptophan. — Proc. Soc. Exp. Biol. Med. 84: 288–291.
Google Scholar - Bevan, E. A. and Makower, M. 1963. The physiological basis of the killer character in yeast. — Proc. Int. Congr. Genet., The Hague. ed. Goerts, S. J. 11: 127.
Google Scholar - Bussey, H. 1972. Effects of yeast killer factor on sensitive cells. — Nature New Biology 235: 73–75.
Google Scholar - Bussey, H. and Skipper, N. 1975. Membrane-mediated killing of Saccharomyces cerevisiae by glycoproteins from Torulopsis glabrata. — J. Bacteriol. 124: 476–483.
Google Scholar - Bussey, H. and Skipper, N. 1976. Killing of Torulopsis glabrata by Saccharomyces cerevisiae killer factor. — Antimicrob. Agents Chemother. 9: 352–354.
Google Scholar - Davies, J. K. and Reeves, P. 1975. Genetics of resistance to colicins in Escherichia coli K-12: Cross-resistance among colicins of group B. — J. Bacteriol. 123: 96–101.
Google Scholar - Fink, G. R. and Styles, C. A. 1972. Curing of a killer factor in Saccharomyces cerevisiae. —Proc. Natl. Acad. Sci. U.S.A. 69: 2846–2849.
Google Scholar - Fraenkel-Conrat, H., Singer, B. and Tsugita, A. 1961. Purification of viral RNA by means of bentonite. — Virology 14: 54–58.
Google Scholar - Franklin, R. M. 1966. Purification and properties of the replicative intermediate of the RNA bacteriophage R17. — Proc. Natl. Acad. Sci. U.S.A. 55: 1504–1511.
Google Scholar - Gault, R. R., Koci, J. and Brockwell, J. 1974. A device for the formation of wells in agar gels. — Lab. Pract. 1974: 11–14.
Google Scholar - Haynes, W. C., Wickerham, L. J. and Hesseltine, C. W. 1955. Maintenance of cultures of industrially important microorganisms. — Appl. Microbiol. 3: 361–368.
Google Scholar - Lodder, J. 1970. The Yeasts: A taxonomic study, 2nd. ed. — North Holland Publ. Co., Amsterdam.
Google Scholar - Loening, U. E. 1987. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. — Biochem. J. 102: 251–257.
Google Scholar - Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. — J. Biol. Chem. 193: 265–275.
Google Scholar - Naumov, G. I. and Naumova, T. I. 1973. Comparative genetics of yeast. XIII. Comparative study of killer strains of Saccharomyces from different collections. — Genetika 9: 140–145.
Google Scholar - Philliskirk, G. and Young, T. W. 1975. The occurrence of killer character in yeasts of various genera. — Antonie van Leeuwenhoek 41: 147–151.
Google Scholar - Puhalla, J. E. 1968. Compatibility reactions on solid media and interstrain inhibition in Ustilago maydis. — Genetics 60: 461–474.
Google Scholar - Shatkin, A. J., Sipe, J. D. and Loh, P. 1968. Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. — J. Virol. 2: 986–991.
Google Scholar - Tzen, J. C., Somers, J. M. and Mitchell, D. J. 1974. A ds RNA analysis of suppressive sensitive mutants of “killer” Saccharomyces cerevisiae. — Heredity 33: 132.
Google Scholar - Vodkin, M., Katterman, F. and Fink, G. R. 1974. Yeast killer mutants with altered doublestranded ribonucleic acid. — J. Bacteriol. 117: 681–686.
Google Scholar - Wickner, R. B. 1974. “Killer character” of Saccharomyces cerevisiae: curing by growth at elevated temperatures. — J. Bacteriol. 117: 1356–1357.
Google Scholar - Wickner, R. B. 1976. Killer of Saccharomyces cerevisiae: a double-stranded ribonucleic acid plasmid. — Bacteriol. Rev. 40: 757–773.
Google Scholar - Woods, D. R. and Bevan, E. A. 1968. Studies on the nature of the killer factor produced by Saccharomyces cerevisiae. — J. Gen. Microbiol. 51: 115–126.
Google Scholar
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Authors and Affiliations
- Department of Biochemistry, University of Birmingham, P. O. Box 363, B15 2TT, Birmingham, England
T. W. Young & M. Yagiu
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- T. W. Young
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Young, T.W., Yagiu, M. A comparison of the killer character in different yeasts and its classification.Antonie van Leeuwenhoek 44, 59–77 (1978). https://doi.org/10.1007/BF00400077
- Received: 18 October 1977
- Issue Date: March 1978
- DOI: https://doi.org/10.1007/BF00400077