An interleukin-1 receptor antagonist protein protects insulin-producing Beta cells against suppressive effects of interleukin-1β (original) (raw)

Summary

The cytokine interleukin-1β may have an important role in the autoimmune mediated damage of pancreatic Beta cells in insulin-dependent diabetes mellitus. In the present study we have investigated the effects of an interleukin-1 receptor antagonist protein, a blocker of the type I interleukin-1 receptor, on the suppressive actions of recombinant interleukin-1β on insulin-producing cells. Brief exposure (1–2 h) of rat and mouse pancreatic islets to 10 ng/ml recombinant interleukin-1β induced an 70–80% inhibition of insulin response to glucose after 12 h. These effects were completely counteracted by co-incubation with 100 ng/ml interleukin-1 receptor antagonist protein. When rat islets were cultured for 48 h in the presence of recombinant interleukin-1β (5 ng/ml) higher concentrations of interleukin-1 receptor antagonist protein (5000 ng/ml) were required to protect Beta-cell function. Interleukin-1 receptor antagonist protein also counteracted the inhibitory effects of recombinant interleukin-1β on the growth of the rat insulinoma cell line RINm5F. These data suggest that interleukin-1 receptor antagonist protein can protect insulin-producing cells from the deleterious effects of recombinant interleukin-1β, and that these cells possess type I interleukin-1 receptors.

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Authors and Affiliations

  1. Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden
    D. L. Eizirik & S. Sandler
  2. Hypersensitivity Diseases Research, The Upjohn Company, Kalamazoo, Michigan, USA
    D. E. Tracey
  3. Laboratory of Medical Immunology, Medical Department TTA, University Hospital, Copenhagen, Denmark
    K. Bendtzen

Authors

  1. D. L. Eizirik
  2. D. E. Tracey
  3. K. Bendtzen
  4. S. Sandler

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Eizirik, D.L., Tracey, D.E., Bendtzen, K. et al. An interleukin-1 receptor antagonist protein protects insulin-producing Beta cells against suppressive effects of interleukin-1β.Diabetologia 34, 445–448 (1991). https://doi.org/10.1007/BF00403185

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