Comparison of the killer toxin of several yeasts and the purification of a toxin of type K2 (original) (raw)

• 286 Accesses
• 77 Citations
• 3 Altmetric
• Explore all metrics

Abstract

A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were recognized. The culture filtrate of Pichia and Candida showed a much smaller activity than the strains of Saccharomyces. Extracellular killer toxins of 3 types of Saccharomyces were concentrated and partially purified. The pH optimum and the isoelectric point were determined. The killer toxins of S. cerevisiae strain NCYC 738, strain 399 and strain 28 were glycoproteins and had a molecular weight of Mr=16,000. The amino acid composition of the toxin type K2 of S. cerevisiae strain 399 was determined and compared with the composition of two other toxins.

Access this article

Log in via an institution

Subscribe and save

• Starting from 10 chapters or articles per month
• Access and download chapters and articles from more than 300k books and 2,500 journals
• Cancel anytime View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

• Bussey H (1972) Effects of yeast killer factor on sensitive cells. Nature New Biology 235:73–75
  Google Scholar
• Bussey H (1981) Physiology of the killer factor in yeast.Adv Microbial Physiol 22:93–122
  Google Scholar
• Bussey H, Sherman D (1973) Yeast killer factor: ATP leakage and coordinate inhibition of macromolecular synthesis in sensitive cells. Biochim Biophys Acta 298:868–875
  Google Scholar
• De La Pena P, Borros F, Gascon S, Lazo PS, Ramas S (1981) Effect of yeast killer-toxin on sensitive cells of Saccharomyces cerevisiae. J Biol Chem 256:10420–10425
  Google Scholar
• Hara S, Imura Y, Otsuka K (1980) Breeding of useful killer wine yeasts. Am J Enol Viticult 31:28–33
  Google Scholar
• Kreil H, Kieninger H, Teuber M (1976) Killerfaktoren produzierende Hefen. Brauwissenschaft 29:102–110
  Google Scholar
• Makower M, Bevan EA (1963) The inheritance of a killer character in yeast (Saccharomyces cerevisiae). Proceedings 11th Internat Congress Genetics 1:202 (abstract)
  Google Scholar
• Middelbeek EJ, Hermans JHM, Stumm C (1979) Production, purification and properties of a Pichia kluyveri killer toxin. Antonie van Leeuwenhoek J Microbiol Serol 45:437–450
  Google Scholar
• Palfree GE, Bussey H (1979) Yeast killer-toxin. Purification and characterization of the protein toxin from Saccharomyces cerevisiae. Eur J Biochem 93:487–493
  Google Scholar
• Pfeiffer P, Radler F (1982) Purification and characterization of extracellular and intracellular killer toxin of Saccharomyces cerevisiae strain 28. J Gen Microbiol 128:2699–2706
  Google Scholar
• Philliskirk G, Young TW (1975) The occurrence of killer character in yeasts of various genera. Antonie van Leeuwenhoek J Microbiol Serol 41:147–151
  Google Scholar
• Skipper N, Bussey H (1977) Mode of action of yeast toxins: energy requirement for Saccharomyces killer toxin. J Bacteriol 129:668–677
  Google Scholar
• Somers JM, Bevan EA (1969) The inheritance of the killer-character in yeast. Genet Res 13:71–84
  Google Scholar
• Spiro RG (1972) Study of the carbohydrates of glycoproteins. In: SP Colowick, NO Kaplan (eds) Methods in enzymology, vol 28. Academic Press, New York London, pp 3–43
  Google Scholar
• Stumm C, Hermans JHM, Middelbeek EJ, Croes AF, De Vries GJML (1977) Killer-sensitive relationships in yeasts from natural habitats. Antonie van Leeuwenhoek J Microbiol Serol 43:125–128
  Google Scholar
• Toh-e A, Guerry P, Wickner RB (1978) Chromosomal superkiller: mutants of Saccharomyces cerevisiae. J Bacteriol 136:1002–1007
  Google Scholar
• Wickner RB (1981) Killer systems in Saccharomyces cerevisiae. In; JN Strathern, EW Jones, JR Broach (eds) The molecular biology of the yeast Saccharomyces. Life cycle and inheritance.Cold Spring Harbour Laboratory, pp 415–444
• Woods DR, Bevan EA (1968) Studies on the nature of the killer factor produced by Saccharomyces cerevisiae. J Gen Microbiol 51:115–126
  Google Scholar
• Young TW (1981) The genetic manipulation of killer character into brewing yeast. J Inst Brewing 87:292–295
  Google Scholar
• Young TW, Yagiu M (1978) A comparison of killer character in different yeasts and its classification. Antonie van Leeuwenhoek J Microbiol Serol 44:59–77
  Google Scholar

Download references

Author information

Authors and Affiliations

1. Institut für Mikrobiologie und Weinforschung der Johannes Gutenberg-Universität Mainz, Saarstraße 21, D-6500, Mainz, Germany
   P. Pfeiffer & F. Radler

Authors

1. P. Pfeiffer
2. F. Radler

Rights and permissions

About this article

Cite this article

Pfeiffer, P., Radler, F. Comparison of the killer toxin of several yeasts and the purification of a toxin of type K2.Arch. Microbiol. 137, 357–361 (1984). https://doi.org/10.1007/BF00410734

Download citation

• Received: 27 October 1983
• Accepted: 12 December 1983
• Issue date: April 1984
• DOI: https://doi.org/10.1007/BF00410734

Key words