Isolation of yeast mutants sensitive to the bifunctional alkylating agent nitrogen mustard (original) (raw)

Summary

Mutants of Saccharomyces cerevisiae with enhanced sensitivity to the DNA cross-linking agent nitrogen mustard (HN2) have been isolated and partially characterized with respect to their phenotypic and genetic properties. The screening technique, based on HN2-sensitivity as sole criterion, yields approximately 1 sensitive isolate in 200 clones when applied to an intensively mutagenized population of a resistant parent strain. Mutants characterized so far are all due to recessive nuclear genes and represent at least seven complementation groups. They exhibit different degrees as well as different patterns of sensitivity towards monofunctional and bifunctional alkylating agents, and ultraviolet light.

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Authors and Affiliations

1. Institut für Mikrobiologie, Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7, D-6000, Frankfurt/M., Germany
   Axel Ruhland, Eckard Haase, Wolfram Siede & Martin Brendel

Authors

1. Axel Ruhland
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2. Eckard Haase
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3. Wolfram Siede
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4. Martin Brendel
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Communicated by F. Kaudewitz

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Ruhland, A., Haase, E., Siede, W. et al. Isolation of yeast mutants sensitive to the bifunctional alkylating agent nitrogen mustard.Molec. Gen. Genet. 181, 346–351 (1981). https://doi.org/10.1007/BF00425609

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• Received: 08 September 1980
• Issue Date: March 1981
• DOI: https://doi.org/10.1007/BF00425609

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