cir, a gene conferring resistance to colicin I maps between mgl and fpk on the Escherichia coli chromosome (original) (raw)

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Summary

With the help of the tetracycline resistance transposon Tn_10_ in and around the mgl genes the gene cir was mapped. cir is 80% cotransducible with mgl by P1 transduction. The sequence of the surrounding markers in clockwise order was established as: cdd fpk cir mgl gyrA. The direction of transcription in cir was determined as clockwise on the Escherichia coli chromosome. The gene product of cir, an outer membrane receptor for colicin I, is not part of the mgl operon. It is not regulated by D-fucose, the inducer of the mgl system and mutants defective in cir are unimpaired in the uptake of substrates of the _mgl_-dependent transport system.

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Authors and Affiliations

1. Department of Biology, University of Konstanz, P.O. Box 5560, D-7750, Konstanz, Germany
   Winfried Boos, Christine Bantlow, Dorothee Benner & Elke Roller

Authors

1. Winfried Boos
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2. Christine Bantlow
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3. Dorothee Benner
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4. Elke Roller
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Communicated by E. Bautz

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Boos, W., Bantlow, C., Benner, D. et al. cir, a gene conferring resistance to colicin I maps between mgl and fpk on the Escherichia coli chromosome.Molec. Gen. Genet. 191, 401–406 (1983). https://doi.org/10.1007/BF00425754

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• Received: 07 May 1983
• Issue Date: January 1983
• DOI: https://doi.org/10.1007/BF00425754

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