Characterization of the gene urf13-T and an unidentified reading frame, ORF 25, in maize and tobacco mitochondria (original) (raw)

Summary

We have previously identified two large open reading frames, designated ORF13 and ORF25, in the Texas male-sterile cytoplasm (_cms_-T) of maize mitochondrial DNA (mtDNA). ORF13 is a single copy gene of chimeric origin that is uniquely transcribed and translated in the mitochondria of _cms_-T maize, where it produces a polypeptide of approximately 13,000 Mr. The ORF13 reading frame does not occur in the maize N, C or S cytoplasms or Nicotiana tabacum. ORF25 exists as a single copy and is transcribed in the four major maize cytoplasms (N, T, C and S) and N. tabacum. The predicted ORF25 polypeptide has a molecular weight of 24,374 in normal maize and 22,439 in tobacco. Several nucleotide and predicted amino acid changes have occurred in the ORF25 gene among the four maize cytoplasms and N. tabacum. Properties such as transcription and conservation of the sequence between two diverse species suggests that ORF25 encodes a functional plant mitochondrial gene. The ORF25 sequence of maize contains a chloroplast DNA insert homologous to a tRNA-Arg gene; this chloroplast DNA insert is absent in the tobacco ORF25 sequence. Comparison of the ORF25 and ORF13 sequences in restored and non-restored _cms_-T indicates no differences in their nucleotide sequences. Thus fertility restoration does not alter the primary sequences of ORF13 or ORF25.

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Authors and Affiliations

1. Department of Genetics, North Carolina State University, Box 7614, 27695-7614, Raleigh, NC, USA
   S. E. Stamper, R. E. Dewey, M. M. Bland & C. S. Levings III

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1. S. E. Stamper
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2. R. E. Dewey
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3. M. M. Bland
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4. C. S. Levings III
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Stamper, S.E., Dewey, R.E., Bland, M.M. et al. Characterization of the gene urf13-T and an unidentified reading frame, ORF 25, in maize and tobacco mitochondria.Curr Genet 12, 457–463 (1987). https://doi.org/10.1007/BF00434824

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• Received: 29 May 1987
• Issue Date: October 1987
• DOI: https://doi.org/10.1007/BF00434824

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