Molecular cloning of ribosomal protein genes from MYcoplasma capricolum (original) (raw)

Summary

A _Bgl_II-fragment from the Mycoplasma, capricolum DNA cloned into pBR322 has been found to contain a cluster of ribosomal protein genes. The recombinant plasmid, pMCB1088, includes a 9 kilobase-pair insert that codes for at least eight ribosomal proteins of M. capricolum. The protein genes are expressed in Escherichia coli cells.

Access this article

Log in via an institution

Subscribe and save

• Starting from 10 chapters or articles per month
• Access and download chapters and articles from more than 300k books and 2,500 journals
• Cancel anytime View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

• Bolivar F, Backman K (1979) Plasmids of Escherichia coli as cloning vectors. Methods Enzymol 68:245–267
  Google Scholar
• Casadaban M, Cohen S (1980) Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol 138:179–207
  Google Scholar
• Hardy SJS, Kurland CG, Voynow P, Mora G (1969), The ribosomal proteins of Escherichia coli. I. Purification of 30S ribosomal proteins. Biochemistry 8:2897–2905
  Google Scholar
• Kaltschmidt E, Wittmann HG (1970) Ribosomal proteins VII. Two dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem 36:402–412
  Google Scholar
• Kawauchi Y, Muto A, Osawa S (1982) The protein composition of Mycoplasma capricolum. Mol Gen Genet 188:7–11
  Google Scholar
• Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
  Google Scholar
• Nomura M, Post LE (1980) Organization of ribosomal genes and regulation of their expression in Escherichia coli. In: Chambliss G, Craven GR, Davies J, Davis K, Kahan L, Nomura M (eds) Ribosomes. University Park Press, Baltimore, Maryland, 671–691
  Google Scholar
• Oka A, Sugisaki H, Takanami M (1981) Nucleotide sequence of the kanamycin resistance transposon Tn903. J Mol Biol 147:217–226
  Google Scholar
• Osawa S (1982) Ribosomal genes in Bacillus subtilis Comparison with Escherichia coli. Microbiology-1982: 19–21
• Razin S (1978) The Mycoplasmas. Microbiol Rev 42:414–470
  Google Scholar
• Ryan JH, Morowitz HJ (1969) Partial purification of native rRNA and tRNA cistrons from Mycoplasma sp. (KID). Proc Natl Acad Sci USA 63:1282–1289
  Google Scholar
• Sancer A, Hack AM, Rupp WD (1979) Simple method for identification of plasmid-coded proteins. J Bacteriol 137:692–693
  Google Scholar
• Sawada M, Muto A, Yamao F, Iwami M, Osawa S (1984) Organization of ribosomal RNA genes in Mycoplasma capricolum. Mol Gen Genet 196:311–316
  Google Scholar
• Vogelstein B, Gillespie D (1979) Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci USA 76:615–619
  Google Scholar
• Wallace DC, Morowitz HJ (1973) Genome size and evolution Chromosoma 40:121–126
  Google Scholar

Download references

Author information

Author notes

1. Yasushi Kawauchi
   Present address: Department of Biochemistry and Biophysics, Research Institute for Nuclear Medicine and Biology, Hiroshima University, 734, Hiroshima, Japan

Authors and Affiliations

1. Laboratory of Molecular Genetics, Department of Biology, Faculty of Science, Nagoya University, 464, Nagoya, Japan
   Yasushi Kawauchi, Akira Muto, Fumiaki Yamao & Syozo Osawa

Authors

1. Yasushi Kawauchi
2. Akira Muto
3. Fumiaki Yamao
4. Syozo Osawa

Additional information

Communicated by K. Isono

Rights and permissions

About this article

Cite this article

Kawauchi, Y., Muto, A., Yamao, F. et al. Molecular cloning of ribosomal protein genes from MYcoplasma capricolum.Molec. Gen. Genet. 196, 521–525 (1984). https://doi.org/10.1007/BF00436202

Download citation

• Received: 18 May 1984
• Issue date: September 1984
• DOI: https://doi.org/10.1007/BF00436202

Keywords