A new bacterial gene (groPC) which affects λ DNA replication (original) (raw)
Summary
A bacterial mutation affecting λ DNA replication, called _groP_C756, has been mapped between the thr and leu bacterial loci. Most of the parental λ DNA does not undergo even one round of replication in this host. Lambda mutants, called π, which map in the λ P gene are able to overcome the inhibitory effect of the _groP_C756 mutation. It is shown that the mutation at the _groP_C locus also interferes with bacterial growth at 42°C. A λ-transducing phage, carrying the _groP_C+ allele, was isolated as a plaqueformer on _groP_C756 bacteria. Upon lysogenization, it restores both the gro + and temperature resistant phenotypes.
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- C. P. Georgopoulos
Present address: Department of Microbiology, College of Medicine, University of Utah, 84112, Salt Lake City, Utah, USA
Authors and Affiliations
- Department de Biologie Moléculaire, Université de Genève, CH-1211, Genève, Switzerland
C. P. Georgopoulos
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- C. P. Georgopoulos
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Communicated by F. Stahl
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Georgopoulos, C.P. A new bacterial gene (_groP_C) which affects λ DNA replication.Molec. Gen. Genet. 151, 35–39 (1977). https://doi.org/10.1007/BF00446910
- Received: 12 November 1976
- Issue Date: January 1977
- DOI: https://doi.org/10.1007/BF00446910