Isolation of mutants sensitive to 2-amiriopurine and alkylating agents and evidence for the role of DNA methylation in Penicillium chrysogenum (original) (raw)
Summary
Using high performance liquid chromatography, the presence of N6-methyladenine has been found at a level of 0.1 mol percent in DNA extracted from Penicillium chysogenum. No 5-methylcytosine was detected. A mutant strain HP547, which is sensitive to the lethal effects of N-methyl-N1-nitro-N-nitrosoguanidine, methylmethane sulphonate and the base analogue 2-aminopurine shows an increased spontaneous mutation rate and no detectable DNA methylation. Comparison of restriction enzyme digests of wild type and undermethylated strains indicated that methylation was occurring at a different sequence to that of the Dam methylase system of E. coli.
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References
- Adams RLP, Burdon RH (1982) CRC Crit Rev Biochem 13:349 to 384
Google Scholar - Ditchburn P, Giddings B, Macdonald KD (1974) J Appl Bacteriol 37:515–523
Google Scholar - Ehrlich M, Wang RYH (1981) Science 212:1350–1357
Google Scholar - Glickman B, Radman M (1980) Proc Natl Acad Sci USA 77:1063 to 1066
Google Scholar - Glickman B, van den Elsan P, Radman M (1978) Mol Gen Genet 163:307–312
Google Scholar - Hattman S, Gold E, Plotnik A (1972) Proc Natl Acad Sci USA 69:187–190
Google Scholar - Hattman S, Kenny C, Berger L, Pratt K (1978) J Bacteriol 135:1156–1157
Google Scholar - Holt G, Adlard M, Lisboa E, Rogers ME, Rogers SD, Saunders G, Smith T (1984) Biochem Soc Trans 12:577–580
Google Scholar - Lacks S, Greenberg B (1977) J Mol Biol 114:153–168
Google Scholar - Marinus MG, Morris NR (1973) J Bacteriol 114:1143–1150
Google Scholar - Raper KB, Alexander DF (1945) J Elisha Mitchell Sci Soc 61:74–113
Google Scholar - Rogers SD, Holt G (1984) Appl Microbiol Biotechnol 20:251–255
Google Scholar - Saunders G, Rogers ME, Adlard MW, Holt G (1984) Mol Gen Genet 1984:343–345
Google Scholar - Smith JD, Arber W, Kuhnlein U (1972) J Mol Biol 63:1–8
Google Scholar - Tamane M, Antegueia F, Villaneuva JR, Santos T (1983) J Gen Microbiol 129:2079–2089
Google Scholar - Wagner I, Capesius I (1981) Biochim Biophys Acta 654:52–56
Google Scholar - Whittacker PA, Hardman N (1980) Biochem J 191:859–862
Google Scholar
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- S. D. Rogers & G. Holt
Present address: The Biological Laboratory, The University of Kent at Canterbury, CT2 7NJ, Canterbury, Kent, UK
Authors and Affiliations
- School of Biotechnology, Faculty of Engineering and Science, The Polytechnic of Central London, 115 New Cavendish Street, London, WlM 8JS, UK
S. D. Rogers, M. E. Rogers, G. Saunders & G. Holt
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- S. D. Rogers
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Rogers, S.D., Rogers, M.E., Saunders, G. et al. Isolation of mutants sensitive to 2-amiriopurine and alkylating agents and evidence for the role of DNA methylation in Penicillium chrysogenum.Curr Genet 10, 557–560 (1986). https://doi.org/10.1007/BF00447390
- Received: 01 October 1985
- Issue Date: March 1986
- DOI: https://doi.org/10.1007/BF00447390