Lectin histochemistry of mammalian endothelium (original) (raw)

Summary

Lectin-histochemical studies were performed on formalin-fixed, paraffin-embedded tissues from ten mammalian species to demonstrate the pattern of carbohydrate residues in vascular endothelium. Ten different biotinylated lectins were used as probes and avidin-biotin-peroxidase complex (ABC) was used as visualant. Ricinus communis agglutinin-I (RCA-I) and wheat germ agglutinin (WGA) stained vascular endothelium in all species. Peanut agglutinin (PNA) stained vascular endothelium in all species only after preincubation with neuraminidase. Bandeirea simplicifolia agglutinin-I (BS-I) stained vascular endothelium in all species but human, while, Ulex europeus agglutinin-I (UEA-I) stained only human endothelium. Individual differences in staining of human vascular endothelium were noted with BS-I and succinylated-WGA (SWGA). Similarly, individual differences in staining of animal vascular endothelium were noted with soybean agglutinin (SBA) after preincubation with neuraminidase. Finally, Concanavalia ensiformis agglutinin (Con A), Dolichos biflorus agglutinin (DBA) and Lens culinaris agglutinin (LCA) did not stain vascular endothehuman in any of the species studied.

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Authors and Affiliations

1. Department of Pathology, Tufts University, Schools of Medicine and Veterinary Medicine, 136 Harrison Avenue, 02111, Boston, Massachusetts, USA
   J. Alroy & V. Goyal
2. Sackler School of Medicine, Tel-Aviv University, Tel Aviv, Israel
   E. Skutelsky

Authors

1. J. Alroy
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2. V. Goyal
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3. E. Skutelsky
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Alroy, J., Goyal, V. & Skutelsky, E. Lectin histochemistry of mammalian endothelium.Histochemistry 86, 603–607 (1987). https://doi.org/10.1007/BF00489554

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• Received: 08 September 1986
• Accepted: 04 January 1987
• Issue Date: November 1987
• DOI: https://doi.org/10.1007/BF00489554

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