Lectin binding and surface glycoprotein pattern of human macrophage populations (original) (raw)
Summary
In the present study unstimulated and stimulated human blood monocytes, untreated and phorbol ester treated U-937 cells, as well as human peritoneal and alveolar macrophages were studied with respect to their surface membrane properties. Binding of different lectins and electrophoretic patterns of tritium labeled surface glycoproteins were compared. The analysis of surface glycoproteins could be interpreted as evidence for a common origin of the analysed cell populations. Furthermore, banding patterns of glycoproteins might be useful to define certain activation states within monocyte/macrophage differentiation. In contrast, lectin binding pattern did not clearly discriminate macrophage subpopulations.
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Animal Lectin
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Abbreviations
AM:
alveolar macrophage
BM:
blood monocyte
PM:
peritoneal macrophage
PBS:
phosphate buffered saline
IPA:
12-O-tetradecanoylphorbol-13-acetate
Con A:
Concanavalin A
HPA:
Helix pomatia agglutinin
LPA:
Limulus polyphemus agglutinin
PHA:
Phaseolus vulgaris agglutinin
SBA:
Soy bean agglutinin
UEA I:
Ulex europaeus agglutinin I
WGA:
Wheat-germ agglutinin
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- U. Schumacher
Present address: Anatomische Anstalt (Lehrstuhl II), Pettenkofer Str. 11, D-8000, München 2, Germany
Authors and Affiliations
- Institute of Pathology, University of Kiel, Hospitalstrasse 42, D-2300, Kiel, Germany
H. Kreipe, H. J. Radzun, U. Schumacher & M. R. Parwaresch
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- H. Kreipe
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Kreipe, H., Radzun, H.J., Schumacher, U. et al. Lectin binding and surface glycoprotein pattern of human macrophage populations.Histochemistry 86, 201–206 (1986). https://doi.org/10.1007/BF00493388
- Accepted: 12 June 1986
- Issue Date: March 1986
- DOI: https://doi.org/10.1007/BF00493388