High-voltage starch-gel electrophoresis in the study of post-albumin proteins and C'3 (ß1e-globulin) polymorphism (original) (raw)
Abstract
Improved methods for high-voltage starch-gel electrophoresis were developed, resulting in improved resolutions of post-albumin proteins and observation of an additional component in Gc (group specific component) proteins of phenotypes 1-1 and 2-1. Common inherited differences in the C'3 proteins (ß1c-globulin) of the complement system were discovered with the new systems. Autosomal codominant inheritance of two common alleles (C3 1and C3 2) and three relatively uncommon alleles (C3 3, C3 4, and C3 5) at a single locus was supported by studies of randomly selected sera from 123 Caucasians and 113 Negroes. Frequencies of C'3 genes were: for Caucasions, C3 1=0.21, C3 2=0.77, C3 3≈0.01, C3 4≈0.004, C3 5≈0.004; for Negroes, C3 1=0.04, C3 2=0.96. Studies of 34 families and 99 children supported the proposed autosomal codominant inheritance of C3 1, C3 2, C3 3, and C3 4. The C'3 phenotype was fully expressed in the newborn and sometimes differed from that of the mother, suggesting synthesis of C'3 by the newborn without placental transmission.
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Authors and Affiliations
- Department of Medicine, University of Wisconsin, Madison, Wisconsin
E. A. Azen - Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin
O. Smithies - P.O. Box 1294, Madison, Wisconsin
O. Hiller
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- E. A. Azen
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This study was supported by grants from the National Institutes of Health (AMO 9555-02 and GM 15422) and from the National Science Foundation (GB 4362).
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Azen, E.A., Smithies, O. & Hiller, O. High-voltage starch-gel electrophoresis in the study of post-albumin proteins and C'3 (ß1e-globulin) polymorphism.Biochem Genet 3, 215–228 (1969). https://doi.org/10.1007/BF00521138
- Received: 30 December 1968
- Revised: 22 January 1969
- Issue Date: June 1969
- DOI: https://doi.org/10.1007/BF00521138