Calcium currents in single SA nodal cells of the rabbit heart studied with action potential clamp (original) (raw)

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Abstract

With a new method called “Action Potential-Clamp” (APC) we studied in single SA nodal cells the contribution of both transient and long lasting calcium currents (L-type and T-type) to the action potential. Action potentials were recorded by a computer and a representative cycle was subsequently repetitively replayed to the same cell under voltage clamp. Blockade of the L-type calcium current (D600) or T-type calcium current (nickel) revealed the quantitative and time related contributions of these currents to the action potential, since the blocked current is compensated by the clamp amplifier.

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Authors and Affiliations

1. II. Physiologisches Institut, Universität des Saarlandes, D-6650, Homburg/Saar, Federal Republic of Germany
   Th. Doerr, R. Denger & W. Trautwein

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1. Th. Doerr
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2. R. Denger
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3. W. Trautwein
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Doerr, T., Denger, R. & Trautwein, W. Calcium currents in single SA nodal cells of the rabbit heart studied with action potential clamp.Pflugers Arch. 413, 599 (1989). https://doi.org/10.1007/BF00581808

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• Received: 23 June 1988
• Revised: 11 October 1988
• Accepted: 23 November 1988
• DOI: https://doi.org/10.1007/BF00581808

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