A specific histochemical marker (lectinRicinus communis agglutinin-1) for normal human microglia, and application to routine histopathology (original) (raw)

Summary

Microglia were demonstrated in paraffinembedded human nervous tissues with an avidinbiotin peroxidase method and_Ricinus communis_ agglutinin-1 (RCA-1). Specific staining was observed in cell bodies and processes of microglia. Although endothelial cells and blood cells reacted with RCA-1, they were easily distinguished morphologically from microglia. Astrocytes, oligodendrocytes, and neurons did not react with RCA-1. These results suggest that RCA-1 can be used as a new histochemical marker for microglia in normal human brain.

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Authors and Affiliations

1. Division of Neuropathology, The Hospital for Sick Children, 555 University Avenue, M5G 1X8, Toronto, Ontario, Canada
   H. Mannoji, H. Yeger & L. E. Becker

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1. H. Mannoji
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2. H. Yeger
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3. L. E. Becker
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Mannoji, H., Yeger, H. & Becker, L.E. A specific histochemical marker (lectin_Ricinus communis_ agglutinin-1) for normal human microglia, and application to routine histopathology.Acta Neuropathol 71, 341–343 (1986). https://doi.org/10.1007/BF00688060

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• Received: 17 February 1986
• Accepted: 14 July 1986
• Issue Date: September 1986
• DOI: https://doi.org/10.1007/BF00688060

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