Interaction ofTrypanosoma cruzi with macrophages in vitro: Dissociation of the attachment and internalization phases by low temperature and cytochalasin B (original) (raw)
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Abstract
Chicken macrophages, obtained by cultivation of blood monocytes, were infected with epimastigote and bloodstream trypomastigote forms of_Trypanosoma cruzi_ strain Y. The percentage of macrophages containing parasites within parasitophorous vacuoles and of flagellates attached to cell surfaces was determined. By incubation of the macrophages at 4°C or in the presence of cytochalasin B it was possible to dissociate the attachment from the internalization phases in the process of infection of macrophages. Both treatments had a marked effect on the internalization of epimastigote and trypomastigote forms. Cytochalasin B treatment and placement of the macrophages at 4° C before infection inhibited this process by about 99 and 96%, respectively. These results suggest that endocytosis is the principal mechanism of internalization of_T. cruzi_ by macrophages. They show also that epimastigote and trypomastigote forms of_T. cruzi_ have a different rate of adhesion to the macrophage surface.
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Authors and Affiliations
- Laboratório de Ultra-Estrutura Celular, Instituto de Biofísica, Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21910, Rio de Janeiro, RJ, Brasil
M. N. L. de Meirelles, T. C. de Araújo Jorge & W. de Souza - Fundação Oswaldo Cruz, Avenida Brasil, 21040, Rio de Janeiro, RJ, Brasil
M. N. L. de Meirelles
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- M. N. L. de Meirelles
You can also search for this author inPubMed Google Scholar - T. C. de Araújo Jorge
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de Meirelles, M.N.L., de Araújo Jorge, T.C. & de Souza, W. Interaction of_Trypanosoma cruzi_ with macrophages in vitro: Dissociation of the attachment and internalization phases by low temperature and cytochalasin B.Z. Parasitenkd. 68, 7–14 (1982). https://doi.org/10.1007/BF00926652
- Accepted: 02 February 1982
- Issue Date: February 1982
- DOI: https://doi.org/10.1007/BF00926652