Fine structural relationships between neurites and radial glial processes in developing mouse spinal cord (original) (raw)

Summary

Neurites have been observed in various types of fine structural associations with developing radial glial processes in the lateral marginal zones of embryonic mouse spinal cords. The least complex of the neurite-glial relationships involves a simple apposition of dendrites and dendritic growth cones with radial glial processes. We interpret this relationship to mean that dendrites, growing from the somata of the lateral motor nucleus, may preferentially move along the interface between radial glial processes and the adjacent axons of the marginal zone. A somewhat more specialized neurite-glial association is the occurrence of_puncta adhaerentia_ between neurites and radial glial processes. Finally, the most elaborate morphological relationship observed is that of synapse-like contacts between axons and radial glial processes. These ‘axoglial synapses’ begin to appear at E11 and E12, are most numerous in E13 and E14 spinal cords, but they can not be found after embryonic day 15. Based on these observations, we make the following speculation: the formation of synaptic junctions occurs in a hierarchy of steps with a process of trial and error primarily involved in the initial stages of synapto-genesis, while more specific requirements are involved in the later steps of synaptic differentiation. Thus, the formation of ‘axoglial synapses’ may reflect errors in development while their disappearance may result when more specific mechanisms of synaptic differentiation become operational.

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References

• Boulder Committee (1970) Embryonic vertebrate central nervous system: revised terminology.Anatomical Record 166, 257–62.
  Google Scholar
• Grainger, F. andJames, D. W. (1970) Association of glial cells with the terminal parts of neurite bundles extending from chick spinal cord_in vitro_.Zeitschrift für Zellforschung und mikroskopische Anatomie 108, 93–104.
  Google Scholar
• Grainger, F., James, D. W. andTresman, R. L. (1968) An electron-microscopic study of the early outgrowth from chick spinal cord_in vitro_.Zeitschrift für Zellforschung und mikroskopische Anatomie 90, 53–67.
  Google Scholar
• Guillery, R. W., Sobkowicz, H. M. andScott, G. L. (1970) Relationships between glial and neuronal elements in the development of long term cultures of the spinal cord of the fetal mouse.Journal of Comparative Neurology 140, 1–34.
  Google Scholar
• Henrikson, C. K. andVaughn, J. E. (1974) Synapse-like contacts between axons and glia in the embryonic mouse spinal cord.Anatomical Record 178, 372–73 (Abstract).
  Google Scholar
• Hinds, J. W. andRuffett, T. L. (1971) Cell proliferation in the neural tube: an electron microscopic and Golgi analysis in the mouse cerebral vesicle.Zeitschrift für Zellforschung und mikroskopische Anatomie 115, 226–64.
  Google Scholar
• James, D. W. andTresman, R. L. (1969) Synaptic profiles in the outgrowth from chick spinal cord_in vitro_.Zeitschrift für Zellforschung und mikroskopische Anatomie 101, 598–606.
  Google Scholar
• Nornes, H. O. andDas, G. D. (1972) Temporal pattern of neurogenesis in spinal cord: cytoarchitecture and directed growth of axons.Proceedings of the National Academy of Sciences U.S.A. 69, 1962–66.
  Google Scholar
• Peters, A. andFeldman, M. (1973) The cortical plate and molecular layer of the late rat fetus.Zeitschrift für Anatomie und Entwicklungsgeschichte 141, 3–37.
  Google Scholar
• Rakic, P. (1971) Neuron-glia relationship during granule cell migration in developing cerebellar cortex. A Golgi and electron microscopic study in_Macacus rhesus_.Journal of Comparative Neurology 141, 283–312.
  Google Scholar
• Rakic, P. (1972) Mode of cell migration to the superficial layers of fetal monkey neocortex.Journal of Comparative Neurology 145, 61–84.
  Google Scholar
• Rakic, P. andSidman, R. L. (1973) Sequence of developmental abnormalities leading to granule cell deficit in cerebellar cortex of Weaver mutant mice.Journal of Comparative Neurology 152, 103–132.
  Google Scholar
• Ramon Ý Cajal, S. (1960)Studies in Vertebrate Neurogenesis (translated byGuth, L.). Springfield: Charles C Thomas.
  Google Scholar
• Vaughn, J. E., Henrikson, C. K. andGrieshaber, J. A. (1974) A quantitative study of synapses on motor neuron dendritic growth cones in developing mouse spinal cord.Journal of Cell Biology 60, 664–72.
  Google Scholar
• Weiss, P. (1941) Nerve patterns: the mechanics of nerve growth.Growth (Supplement) 5, 163–203.
  Google Scholar

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Authors and Affiliations

1. Division of Neurosciences, City of Hope National Medical Center, 91010, Duarte, California
   Charles K. Henrikson & James E. Vaughn

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1. Charles K. Henrikson
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2. James E. Vaughn
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Supported by U.S.P.H.S. grant NS 09578 from the National Institute of Neurological Diseases and Stroke,

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Henrikson, C.K., Vaughn, J.E. Fine structural relationships between neurites and radial glial processes in developing mouse spinal cord.J Neurocytol 3, 659–675 (1974). https://doi.org/10.1007/BF01097190

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• Received: 03 June 1974
• Accepted: 20 July 1974
• Issue Date: December 1974
• DOI: https://doi.org/10.1007/BF01097190

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